中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 甘蓝型油菜与芸芥属间杂种F_1的获得及鉴定 中国油料作物学报 2005,27 (4) 7-12
Position: Home > Articles > Regeneration and identification of intergeneric hybrid between Brassica napus and Eruca sativa Chinese Journal of Oil Crop Sciences 2005,27 (4) 7-12

甘蓝型油菜与芸芥属间杂种F_1的获得及鉴定

作  者：
戴林建;李栒;官春云;陈社员;钟军
单  位：
湖南农业大学农学院
关键词：
油菜;芸芥;子房培养;RAPD
摘  要：
常规有性杂交很难获得油菜与芸芥的属间杂种。通过对早期杂种胚胎的子房培养,获得128株小苗。经形态学鉴定,其中24株为真杂种。运用分子标记技术(RAPD)进行真假杂种检测,87%的单株带有芸芥特异的标记带。在培养中,适宜的生长素和细胞分裂素浓度以及恰当的子房离体前的发育时间能提高成胚数和成胚率。
译  名：
Regeneration and identification of intergeneric hybrid between Brassica napus and Eruca sativa
作  者：
DAI Lin-jian,LI Xun,GUAN Chun-yun,CHENG She-yuan,ZHONG Jun(The Department of Agronomy,Hunan Agricultural University,Changsha 410128,China)
关键词：
Brassica napus L.;Eruca sativa;Rocketsalad;Ovary culture;RAPD markers
摘  要：
It is difficult to obtain the intergeneric hybrid between Brassica napus and Eruca sativa through the routine crossing.Ovary culture of hybrid embryos was used at early phase and 128 seedlings were obtained successfully.After morphological observations 24 seedlings were identified as potential hybrid.The analysis of random amplified polymorphic DNA(RAPD) showed 21 out of 24 F_1 hybrid progenies(87 %) carrying Eruca genome-specific RAPD markers,providing further evidences to support the speciation of these hybrids.Suitable hormone and cytokin concentration as well as time for excising ovary were crucial for increasing the number of hybrid embryos and the pecentage of hybrid embryos.

相似文章

导出引用
计量
文章访问数: 7
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46