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Position: Home > Articles > Pathogen Identification and Fungicides Susceptibility on Impatiens walleriana Root and Stem Rot Journal of Northeast Forestry University 2016 (3) 101-105

非洲凤仙茎基腐病病原学及药剂敏感性

作  者:
孙慧颖;陈悦;周默;边雪;韩双;白庆荣
单  位:
吉林农业大学
关键词:
非洲凤仙;茎基腐病;病原鉴定;生物学特性;药剂敏感性
摘  要:
对非洲凤仙(Impatiens wallerana Hook.f.)茎基腐病的症状进行了描述,结合病原形态特征、培养特性及r DNA-ITS序列分析结果,确定该病害由Rhizoctonia solani Kühn AG4-HG-Ⅰ融合群引起。病菌的生物学特性研究表明:菌丝较适生长温度为25~30℃;菌丝生长较佳的培养基为OA和PSA,较佳的碳源为D-(+)麦芽糖、α-乳糖和可溶性淀粉,较佳的氮源为硝酸钾和硝酸钠;p H=7时菌丝生长最好;光照条件对菌丝生长无影响;菌丝致死条件为51℃、10 min。采用生长速率法测定了病菌对30种杀菌剂的敏感性,结果表明,病菌对≥300亿个·g-1内生芽孢杆菌WP、1 000亿个·g-1枯草芽孢杆菌WP、25%肟菌·50%戊唑醇WG、40%氟硅唑EC敏感性较高,对其EC50<1.0 mg·L-1,可以作为田间防治非洲凤仙茎基腐病的首选杀菌剂。
译  名:
Pathogen Identification and Fungicides Susceptibility on Impatiens walleriana Root and Stem Rot
作  者:
Sun Huiying;Chen Yue;Zhou Mo;Bian Xue;Han Shuang;Bai Qingrong;Jilin Agricultural University;
关键词:
Impatiens walleriana;;Root and stem rot;;Pathogen identification;;Biological characteristics;;Laboratory toxicity
摘  要:
We described the symptoms of Impatiens walleriana root and stem rot disease. Based on the morphology,cultural characteristics of the isolates and ITS sequence,we identified the pathogen as Rhizoctonia solani Kühn AG4-HG-I. The results of the biological characteristics of the pathogen showed that the suitable temperature for mycelial growth was 25 ℃-30 ℃. The optimal media for mycelial growth were OA and PSA. D-( +) Maltobiose,α-lactose,soluble starch,potassium nitrate and sodium nitrate were optimal for mycelial growth. The pH of 7 was suitable for mycelial growth. Light had no effect on mycelial growth. The lethal temperature of mycelial was 51 ℃ for 10 min. The susceptibility of the pathogen to 30 fungicides was detected by mycelial growth rate method. The pathogen was more sensitive to Endophytic Bacillus WP,Bacillus Subtilis WP,Trifloxystrobin·Tebuconazole 25%·50% WG,Flusilazole 40% EC,EC50<1.0 mg / L.

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