中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 圆齿野鸦椿枝条抗辣椒疫霉病菌活性成分 福建农林大学学报(自然科学版) 2021 (4) 472-479
Position: Home > Articles > 圆齿野鸦椿枝条抗辣椒疫霉病菌活性成分 Journal of Fujian Agriculture and Forestry University(Natural Science Edition) 2021 (4) 472-479

圆齿野鸦椿枝条抗辣椒疫霉病菌活性成分

作  者：
张耀;林智熠;周文娟;潘镇泽;傅佳蕊;陈景新;倪林;邹双全
单  位：
福建农林大学林学院;福建农林大学植物保护学院
关键词：
圆齿野鸦椿;枝条;化学成分;辣椒疫霉病菌;抗真菌活性;抑菌机制;
摘  要：
对圆齿野鸦椿枝条提取物的抑菌活性成分进行分离、纯化,结合现代波谱学技术对其结构进行鉴定,分别鉴定为（+）-（8R, 7′R, 8′R）-isolariciresinol-9-（6-tris-p-coumaroyl）-O-β-D-glucopyranoside（1）、（7R, 8R）-threo-7, 9, 9′-三羟基-3, 3′-二甲氧基-8-O-4′-新木脂素7-O-α-L-鼠李糖苷（2）、4-氧代芝麻素（3）、tetraketide（4）、19α-羟基熊果酸（5）、紫檀芪（6）、evofolin B（7）、豆甾醇（8）.其中,除化合物4、5、7外,其他成分均为本课题组首次从野鸦椿属分离所得.抑菌活性测定结果显示,化合物4、5、7对辣椒疫霉病菌均具有中等抑制活性,其有效中浓度(EC50)值分别为1.285、0.300、0.906μmol·L-1.辣椒疫霉病菌经19α-羟基熊果酸处理后,菌丝干重减少,细胞膜的通透性增加,菌体内蛋白质含量增多,其保护酶（过氧化氢酶、过氧化物酶、超氧化物歧化酶）活性随着处理时间的延长呈倒"V"型变化趋势.这说明19α-羟基熊果酸对辣椒疫霉病菌具有有效抑制作用.
作  者：
ZHANG Yao;LIN Zhiyi;ZHOU Wenjuan;PAN Zhenze;FU Jiarui;CHEN Jingxin;NI Lin;ZOU Shuangquan;College of Plant Protection,Fujian Agriculture and Forestry University;College of Forestry,Fujian Agriculture and Forestry University;Fujian Colleges and Universities Engineering Research Institute of Conservation &Utilization of Natural Bioresources;
关键词：
Euscaphis konishii;;twigs;;chemical constituents;;Phytophthora capsici;;anti-fungal activity;;anti-fungal mechanism
摘  要：
In order to investigate the active constituents of Euscaphis konishii Hayata against Phytophthora capsici, chemical constituents extracted from the anti-fungal active part of E.konishii twig were isolated, purified and further identified by modern spectral analysis techniques. The chemicals were identified as(+)-(8R, 7′R, 8′R)-isolariciresinol-9-(6-tris-p-coumaroyl)-O-β-D-glucopyranoside,(7R, 8R)-threo-7, 9, 9′-trihydroxy-3, 3′-dimethoxy-8-O-4′-lignans7-O-α-L-rhamnoside, 4-oxysesamin, tetraketide, pomolic acid, pterostilbene, evofolin B and stigmasterol. Except for tetraketide, pomolic acid and evofolin B, all other compounds were isolated from this genus for the first time by our research group. Anti-fungal activity testing showed that compounds tetraketide, pomolic acid and evofolin B exerted moderate inhibitory activities against P.capsici, with the EC_(50) value being 1.285, 0.300 and 0.906 μmol·L~(-1), respectively. Furthermore, hyphae dry weight, relative electrical conductivity, contents of malondialdehyde and protein, and activities of protective enzymes(CAT, POD, SOD) of the mycelia treated with pomolic acid were determined to elucidate the anti-fungal mechanism. The results showed that hyphae dry weight was decreased, permeability of cell membrane was enhanced and protein content of the mycelia increased when treated with pomolic acid. The contents of protective enzymes showed inverted V-shaped variation trends with the extension of treatment time. Therefore, pomolic acid had an effective inhibition on P.capsici, which provided a theoretical basis for the development of plant-derived fungicides.

相似文章

导出引用
计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46