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当前位置: 首页 > 文章 > 蓝塘猪和长白猪leptin和leptin受体基因表达的变化 华中农业大学学报 2010,29 (1) 73-76
Position: Home > Articles > Changes of Leptin and Leptin Receptor Gene Expression in Subcutaneous Fat and Hypothalamus of Lantang and Landrace Pigs Journal of Huazhong Agricultural University 2010,29 (1) 73-76

蓝塘猪和长白猪leptin和leptin受体基因表达的变化

作  者：
陈赞谋;戚晓鸿;张豪;吴珍芳;刘德武
单  位：
华南农业大学动物科学学院
关键词：
猪;leptin;leptin受体;基因表达
摘  要：
用实时荧光定量PCR方法研究了蓝塘猪和长白猪1、27、90、150和180日龄阶段脂肪组织中leptin基因mRNA和下丘脑中leptin受体基因mRNA的变化,并对2个品种不同生长阶段血液中leptin含量的变化规律进行了探讨。结果表明:(1)蓝塘猪180日龄时皮下脂肪中leptin基因表达量显著高于其它各阶段,而与长白猪相比,蓝塘猪除1日龄低于长白猪外,其它各个阶段均高于长白猪,180日龄两者差异极显著;长白猪各阶段间差异不显著;(2)蓝塘猪180日龄时下丘脑中leptin受体基因的表达量显著高于其它各阶段,长白猪180日龄时最高,且显著高于1日龄和27日龄,蓝塘猪与长白猪1日龄时差异显著;(3)蓝塘猪血清中leptin浓度各阶段均高于长白猪,两品种均是150日龄时最高,蓝塘猪150日龄显著高于27日龄和180日龄,长白猪各阶段差异不显著。
译  名：
Changes of Leptin and Leptin Receptor Gene Expression in Subcutaneous Fat and Hypothalamus of Lantang and Landrace Pigs
作  者：
CHEN Zan-mou QI Xiao-hong ZHANG Hao WU Zhen-fang LIU De-wu College of Animal Science,South China Agricultural University,Guangzhou 510642,China
关键词：
pig;leptin;leptin receptor;gene expression
摘  要：
Real time RT-PCR was applied to study the developmental patterns of leptin and leptin receptor gene mRNA expression at the ages of 1,27,90,150 and 180 days after born(DAB) in subcutaneous fat and hypothalamus of Lantang and Landrace pigs.The results showed that:(1) in subcutaneous fat,Lantang had the highest leptin mRNA level at 180 DAB.Leptin mRNA at all ages in lantang was higher than that in landrace,except at 1 DAB.Leptin mRNA was significantly higher in Lantang pig than that in landrace pig at 180 DAB.Leptin mRNA had no significant difference among landrace pigs at all ages.(2) In hypothalamus,leptin receptor mRNA was highest at 180 DAB for both the two breeds,and it had significantly higher than that at other ages in lantang,and at 1 and 27 d in landrace.Two breeds at 1 d had apparent differed.(3) Lantang pigs at all ages had higher serum leptin level than landrace.Two breeds at 150 d were the highest compared with other ages.Lantang pigs at 150 day had significantly higher than at ages of 27 and 180 day.No significant changes were seen in all ages in landrace.

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