中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 鸭副粘病毒强毒株的分离和鉴定 中国动物检疫 2001,18 (10)
Position: Home > Articles > Isolation and Identification of High Pathogenic Duck Paramyxovirus China Animal Health Inspection 2001,18 (10)

鸭副粘病毒强毒株的分离和鉴定

作  者：
张训海;朱鸿飞;陈溥言;韩北芳;王旋;赵家龙;朱志海;孙素强
单  位：
凤阳县畜牧兽医站 凤阳 233100;南京农业大学;凤阳县畜牧兽医站;安徽技术师范学院
关键词：
鸭;副粘病毒;分离
摘  要：
采用鸡胚接种法从安徽凤阳某患病肉鸭群中分离到一株病毒。该毒株对鸡红细胞具有凝集性，并可被康复鸭鸭血清和ＮＤＶ阳性抗血清所抑制，而不能被禽流感标准阳性血清抑制，结合血清中和鸡胚接种试验。病毒回归试验和免疫防治效果的研究结果，确认为鸭副粘病毒。参照国际上规定的新城疫病毒毒力判定的标准及其方法，测定该分离株的鸡胚最小致死量平均死亡时间（ＭＤＴ）、１日龄鸡脑内接种致病指数（ＩＣＰＩ）和６周龄鸡静脉内接种致病指数（ＷＰＩ）分别为４８．９ｈ、１．８０和２．４５。表明该鸭副粘病毒分离株具有新城疫病毒（ＮＤＶ）速发型相类似的毒力，属强毒力毒株，并定名为ＷＦ００Ｄ株。
译  名：
Isolation and Identification of High Pathogenic Duck Paramyxovirus
作  者：
Zhang Xunhai, Zhu Hongfei, Chen Puyan, Han Beifang, Wang Xuan， zhao Jialong, Zhu Zhihai, Sun Suqiang (Anhui Technical Teachers College,Fengyang 233100; 2) Nanjing Agricultural University,Nanjing 210095; 3) Animal Husbandry and Veterinary Service Center o
关键词：
Duck Paramyxovirus Isolation
摘  要：
A new viral disease of duck was reported. the strain virus was isolated from infected duck's livers, spleens and pancreas in Fengyang Anhui Province using chicken embryos. The virus could agglutinate the chicken erythrocytes. The hemaagglutination of virus could be inhibited by the convalescent antiserum, the Newcastle disease virus(NDV) F48E9 strain antiserum, the antiserum of goose paramyxovirus type-I isolated by our laboratory respectivcly. But it could' t be inhibited by avian influenza virus(AIV) positive anti- serum. The HA titer ranged between 2~8 and 2~9. Artificial infection could cause up to 100% morbidity and 50% - 100% mortality in 10-day--old ducks, with almost the same clinical symptoms and pathological changes. The 30 - day - old chickens can also be infected with 100% morbidity and 100% mortality. The inactivated vaccine made from the isolated virus and the egg yolk antibodies from its hyperimmunized layers could give very good protection against the natural infection of ducks. The biological characteristics of the virus was determined by measuring the mean death time of chicken embryos(MDT), the intracerebral pathogenicity index in day - old chicks (ICPI) and the intravenous pathogenicity index in 6-week - old chickens(IVPI). The MDT.ICPI and IVPI were 48.9h. 1.8O and 2.45 respectively. The results indicated that the isolate was of high virulence like velogenic Newcastle disease virus(NDV) and named duck paramyxovirus WF00D strain.

相似文章

导出引用
计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46