当前位置: 首页 > 文章 > 高速逆流色谱法分离前胡提取物中香豆素类成分 湖南农业大学学报(自然科学版) 2011,37 (1) 90-93
Position: Home > Articles > Separation of coumarins with function from extract of radix peucedani through HSCCC Journal of Hunan Agricultural University(Natural Sciences) 2011,37 (1) 90-93

高速逆流色谱法分离前胡提取物中香豆素类成分

作  者:
蔡海林;杜良伟;刘祥英;陈桂华;王彦辉;柏连阳
单  位:
湖南农业大学;广西大学
关键词:
白花前胡;高速逆流色谱;前胡香豆素Ⅱ;北美芹素;白花前胡甲素;补骨脂素
摘  要:
采用超临界CO2流体萃取中药前胡中总香豆素,以石油醚∶乙酸乙酯为洗脱系统,对提取物进行硅胶柱初步分离,利用高效液相色谱确定石油醚∶乙酸乙酯∶甲醇∶水=5∶5∶5∶4作为高速逆流色谱(HSCCC)分离的溶剂体系,在主机转速为850 r/min、流动相流速为2.0 mL/min、254 nm波长检测条件下,根据色谱图手动收集各色谱峰组分,分离所得化合物通过核磁共振(NMR)检测并确定其结构,得到4个单体化合物,分别是前胡香豆素Ⅱ、北美芹素、白花前胡甲素、补骨脂素。
译  名:
Separation of coumarins with function from extract of radix peucedani through HSCCC
作  者:
CAI Hai-lin1,DU Liang-wei2,LIU Xiang-ying1,CHEN Gui-hua1,WANG Yan-hui1,BAI Lian-yang1,3(1.College of Bio-Safety Science and Technology,Hunan Agricultural University,Changsha 410128,China;2.School of Chemistry and Chemical Engineering,Guangxi University,Nanning 530004,China;3.Hunan Institute of Humanities Science and Technology,Loudi,Hunan 417000,China)
关键词:
Peucedanum praeruptorum;high speed counter current chromatography;peucedanocoumarin Ⅱ;pteryxin;praeruptorin A;psoralen
摘  要:
With the extract obtained from radix peucedani by SFE(supercritical fluid extraction),coumarins were primarily purifed through silica-gel chromatography with solvent system of petroleum ether:ethyl acetate and further purification was processed by HSCCC(high speed counter current chromatography) with solvent system of petroleum ether:ethyl acetate:methanol:water(5∶5∶5∶4) at the flow rate of 2.0 mL/min under rotator speed of 850 r/min.Fractions were collected manually according to HSCCC chromatogram and then charaterized by NMR(nuclear magnetic resonance),which were peucedanocoumarin II,pteryxin,praeruptorin A and psoralen respectively.

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