作 者：
刘红;曾志杰;李传勇;张水华;孙乐常;陈琼;曹敏杰;刘光明
单 位：
集美大学食品与生物工程学院;福建医科大学福建省新药安全性评价中心;厦门市农产品质量安全检验测试中心
关键词：
4-氯苯氧乙酸钠;急性毒性;蓄积性毒性;超高效液相色谱;毒豆芽
摘 要：
目的:探讨毒豆芽中常用植物生长调节剂4-氯苯氧乙酸钠(sodium 4-chlorophenoxyacetate,4-CPANa)对小鼠的急性、蓄积性毒性及其在小鼠机体的残留规律。方法:采用改良寇氏法测定4-CPANa对小鼠的急性毒性;蓄积性毒性实验以107.4 mg/kg为起始剂量,采用剂量递增蓄积系数法染毒,观察记录实验期间小鼠的一般生理指标,结束后测定小鼠血液生化指标、脏器指数、组织病理变化状况,超高效液相色谱法测定4-CPANa在小鼠机体内的残留量。结果:4-CPANa对小鼠经口半数致死剂量(LD50)为1 074.1 mg/kg,蓄积系数K>8;4-CPANa对小鼠的生理及血液生化指标有不同程度影响,肝脏、肾脏均发生组织病理学变化;小鼠机体中4-CPANa残留量由高到低的顺序为:尿液>肾脏>肝脏>血液>心脏>脑组织>肌肉。结论:4-CPANa为低毒、低等蓄积性药物,其毒性效应主要表现为对小鼠肝脏和肾脏的毒性作用。
译 名：
Acute and Accumulative Toxicity and Detection of Residual Sodium 4-Chlorophenoxyacetate in Mice
作 者：
LIU Hong;ZENG Zhijie;LI Chuanyong;ZHANG Shuihua;SUN Lechang;CHEN Qiong;CAO Minjie;LIU Guangming;College of Food and Biological Engineering, Jimei University;Xiamen Agriculture Product Quality and Safety Testing Center;Fujian Center for Safety Evaluation of New Drug, Fujian Medical University;
关键词：
sodium 4-chlorophenoxyacetate;;acute toxicity;;accumulative toxicity;;ultra-high-performance liquid chromatography(UPLC);;poisonous bean sprout
摘 要：
Objective: To investigate acute and accumulative toxicity and residual patterns of sodium 4-chlorophenoxyacetate(4-CPANa), a plant growth regulator usually abused in poisonous bean sprouts, in mice. Methods: The oral acute toxicity of 4-CPANa in mice was determined by a modified Korbor method, and oral accumulative toxicity was assayed by an incremental exposure accumulative coefficient method with an initial dose of 107.4 mg/kg. Physiological indices of mice were recorded during the experimental period. Serum biochemical and organ indices and morphological examination of mice were carried out, and the 4-CPANa residues in mouse body were analyzed by ultrahigh-performance liquid chromatography at the end of the experiment. Results: The half-lethal dose(LD50) of 4-CPANa to mice was 1 074.1 mg/kg and the accumulative coeffi cient K was larger than 8. Compared with the control group, 4-CPANa showed different infl uence on physiological and serum biochemical indices in mice. Furthermore, 4-CPANa also resulted in visible lesions and significant histopathological changes in the liver and kidney of mice. The 4-CPANa residue in mice was observed in the following decreasing order: urine > kidney > liver > blood > heart > brain > muscle. Conclusion: 4-CPANa was classifi ed as the 4th level of toxicity and belonged to the low-accumulation family. The toxic effect of 4-CPANa toward mice was mainly exhibited as lesions in liver and kidney.
