Position: Home > Articles > Hexafluorobisphenol A can cause oxidative stress in zebrafish liver cells
Modern Journal of Animal Husbandry and Veterinary Medicine
2022
(1)
16-18
六氟双酚A对斑马鱼肝脏细胞氧化应激的影响
作 者：
洪煜宇;杨慧山;谭伟军
单 位：
广东省清远市动物疫病预防控制中心;佛山科学技术学院
关键词：
六氟双酚A;斑马鱼;肝脏细胞;氧化应激;凋亡
摘 要：
为研究六氟双酚A (BPAF)对斑马鱼肝脏细胞的毒性机制。试验以斑马鱼肝脏细胞为研究模型,选择不同浓度的BPAF (0、10、20、30μmol/L)作用于斑马鱼肝脏细胞系ZFL细胞,检测BPAF对ZFL细胞的细胞毒性以及细胞的抗氧化酶活性。结果表明,10、20、30μmol/L BPAF均可极显著抑制ZFL细胞的细胞活性(P<0.01);与对照组相比,10μmol/L BPAF组细胞丙二醛(MDA)含量显著提高(P<0.05),20、30μmol/L BPAF组细胞MDA含量极显著提高(P<0.01);20、30μmol/L BPAF组细胞谷胱甘肽过氧化物酶(GSH-Px)活性极显著降低(P<0.01);10、20、30μmol/L BPAF组细胞过氧化氢酶(CAT)活性极显著降低(P<0.01);20、30μmol/L BPAF组ZFL细胞超氧化物歧化酶(SOD)活性极显著降低(P<0.01)。研究表明,BPAF可通过干扰细胞的氧化应激,对ZFL细胞产生细胞毒性。
译 名：
Hexafluorobisphenol A can cause oxidative stress in zebrafish liver cells
作 者：
Hong Yuyu;Yang Huishan;Tan Weijun;Qingyuan Animal Disease Prevention Control Center;Foshan University;
关键词：
BPAF;;Zebrafish;;Liver cells;;Oxidative stress;;Apoptosis
摘 要：
The experiment was to study the toxic mechanism of BPAF zebrafish liver cells. The experiment used zebrafish liver cells as a research model, different concentrations of hexafluorobisphenol A(BPAF)(0, 10, 20, 30 μmol/L) were selected to act on ZFL cells of the zebrafish liver cell line, detected the cytotoxicity of BPAF to ZFL cells and the activity of intracellular antioxidant enzymes. The results showed that 10, 20, and 30 μmol/L BPAF can extremely inhibit the cell viability of ZFL cells(P<0.01). Compared with the control group, the content of malondialdehyde(MDA) in the cells of the10 μmol/L BPAF group was significantly increased(P<0.05), the 20 and 30 μmol/L BPAF group were extremely increased(P<0.01). The glutathione peroxidase(GSH-Px) activity in the 20 and 30 μmol/L BPAF groups were extremely reduced(P<0.01). The catalase(CAT) activity in the 10, 20, and 30 μmol/L BPAF groups were extremely reduced(P<0.01). The superoxide dismutase(SOD) activity of ZFL cells in the 20 and 30 μmol/L BPAF groups were extremely reduced(P<0.01). Studies have show that BPAF can cause cytotoxicity to ZFL cells by interfering with oxidative stress in the cell.
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