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Position: Home > Articles > Analysis T>C Polymorphisms Located in the Seed Regions of Mir-1644 Gene of Chicken Scientia Agricultura Sinica 2011,44 (13) 2808-2813

鸡mir-1644基因“seed”区的T>C多态分析

作  者：
耿立英;张传生;尹春光;曹顶国;杜立新;陈娟
单  位：
中国农业科学院北京畜牧兽医研究所;河北科技师范学院;济宁学院;山东省农业科学院家禽研究所
关键词：
鸡;mir-1644;seed;单核苷酸多态
摘  要：
【目的】观察"seed"区T>C多态对鸡mir-1644基因"茎环"结构和靶基因选择的影响,分析其在不同鸡群的遗传分布,为揭示其对mir-1644表达调控的影响及表型效应奠定基础。【方法】利用mfold和miRanda软件进行mir-1644二级结构模拟和靶基因预测,采用引入酶切位点RFLP技术对6个鸡群的179个个体进行基因型检测。【结果】①T→C突变可导致pre-mir-1644空间构型和自由能改变,稳定性降低;②预测到21个mir-1644-T和mir-1644-C基因差异调控的靶基因。③在汶上芦花鸡群体内以C等位基因为优势基因,与其它鸡间的基因型分布存在显著差异(P<0.01)。【结论】"seed"区T>C突变可能干扰或破坏mir-1644基因的表达调控,且CC基因型可能与汶上芦花鸡某些表型变异有关。
译  名：
Analysis T>C Polymorphisms Located in the Seed Regions of Mir-1644 Gene of Chicken
作  者：
GENG Li-ying1,ZHANG Chuan-sheng1,YIN Chun-guang2,CAO Ding-guo3,DU Li-xin4,CHEN Juan1(1Hebei Normal University,Changli 066600,Hebei;2Jining College,Jining 273155,Shandong;3Institute of Poultry Science of Shandong Academy of Agricultural Sciences,Ji'nan 250023;4Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193)
关键词：
chicken;mir-1644;seed;a single nucleotide polymorphism
摘  要：
【Objective】 This experiment was conducted to study the effect of T>C polymorphisms located in the seed regions of mir-1644 on its stem-loop secondary structure and target selection,and the differences of distribution of this polymorphisms were also detected in different chicken breeds.【Method】The secondary structures of different alleles of mir-1644 and their target genes were predicted using mfold and miRanda software respectively.CRS-RFLP technique was used to detected chicken mir-1644 genotype in 6 populations containing 179 individuals.【Result】 The results showed that(1) The T→C mutation results in pre-mir-1644 secondary structure change and the free energy of pre-mir-1644-C is higher than that of pre-mir-1644-T;(2) There were 21 target gene differentially regulated by mir-1644-C and mir-1644-T were predicted;(3) Allele C was the dominant allele in Wenshanluhua chicken population,while allele T was the dominant allele in Jiningbairi,Langya,Wugu and populations.The allele and genotype distribution in Wenshangluhua chicken was significantly different with that in other chicken populations(P<0.01).【Conclusion】 These results indicated that T→C mutation may destroy and alter expression and regulation of mir-1644,and CC genotype may be related to some phenotypic variation of Wenshangluhua Chicken.

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