Position: Home > Articles > Production and Characterization of Monoclonal Antibodies against Clostridium Perfringens Type A Enterotoxin
China Animal Husbandry & Veterinary Medicine
2006,33
(9)
63-65
抗A型产气荚膜梭菌单克隆抗体的研制和鉴定
作 者：
张红英;杨霞;陈丽颖;王亚宾;金钺;郑杰;卢中华
单 位：
河南农业大学
关键词：
产气荚膜梭菌肠毒素;单克隆抗体
摘 要：
本试验用N So细胞和产气荚膜梭菌肠毒素免疫的BALB/C小鼠脾细胞融合,获得了2株能分泌抗A型产气荚膜梭菌肠毒素单克隆抗体的杂交瘤细胞。经鉴定,这2株杂交瘤细胞的平均染色体数目为96.8条,单抗亚类为IgG 1,杂交瘤细胞培养上清及腹水单克隆抗体的效价均较高,而且与其他几种常见的毒素SE、LT、S-LT、产气荚膜梭菌α毒素等不存在交叉反应。1D 5能完全中和NCTC 8239、S020718所产生的CPE的生物学活性,而2B 4的中和活性则较差。
译 名：
Production and Characterization of Monoclonal Antibodies against Clostridium Perfringens Type A Enterotoxin
作 者：
ZHANG Hong-ying,YANG Xia,CHENG Li-ying,WANG Ya-bin,JIN Yue,ZHENG Jie,LU Zhong-hua(Henan Agricultural University,Zhenzhou 450002,China)
关键词：
clostridium perfringens enterotoxin;monoclonal antibody
摘 要：
Hybridomas secreting monoclonal antibodies(McAbs)specific for Clostridium perfringens type A enterotoxin(CPE) were produced by fusion of Nso myelomas cells with spleen cells from BALB/C mice immunized with purified CPE.Wells containing hybridomas secreting immunoglobulin G(IgG) antibodies against CPE were specifically identified by an indirect enzyme-linked immunosorbent assay(ELISA),and two strains of ELISA-positive hybridomas were selected and cloned forth by limited dilution.All two hybridomas produced McAbs containing immunoglobulin G1 heavy chains.These hybridomas were then grown as ascitic tumors in mice,and McAbs were purified from the ascites fluids with DEAE.The specificity of the McAbs was demonstrated by RPLA and ELISA.One of the hybridomas with 96.8 average number of chromosome has the activity of neutralizing CPE.
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