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当前位置: 首页 > 文章 > 攸县油茶胚状体发生及其组织学观察 安徽农业科学 2008,36 (8) 3203-3204
Position: Home > Articles > Study on Somatic Embryogenesis and Histological Observation of Camellia yuhsienensis Hu Journal of Anhui Agricultural Sciences 2008,36 (8) 3203-3204

攸县油茶胚状体发生及其组织学观察

作  者：
彭秋发;戴亚娟;杜跃强;吕洪飞
单  位：
浙江师范大学生化学院;浙江省金华市林业局
关键词：
攸县油茶;胚状体;组织培养;组织学观察
摘  要：
[目的]研究以攸县油茶(Camellia yuhsienensis Hu)成熟子叶为外植体的胚状体发生情况。[方法]以子叶为外植体,接种于MS+2.0mg/L2,4-D+1.0mg/LKT培养基上,诱导胚性愈伤组织。胚性愈伤组织块转接至以MS为基本培养基,添加不同浓度6-BA和NAA的激素组合的培养基上诱导产生胚状体。取不同发育阶段的组织块制作石蜡切片,进行组织学观察。[结果]成熟子叶块接种于诱导培养基MS+2.0mg/L2,4-D+1.0mg/LKT,15d左右开始出现胚性愈伤组织。胚性愈伤组织转接至培养基为MS+2.0mg/L6-BA+0.6mg/LNAA时胚状体诱导率最高,为87.78%;无激素培养基也能诱导胚性愈伤组织产生胚状体,但诱导率极低,仅为6.67%。组织学观察表明,胚性细胞由愈伤组织表面及其内部邻近的若干细胞分化而来,经过球形胚、心形胚、子叶形胚等不同发育阶段直至成熟胚状体。[结论]诱导胚性愈伤组织分化为胚状体的最佳培养基为MS+2.0mg/L6-BA+0.6mg/LNAA。
译  名：
Study on Somatic Embryogenesis and Histological Observation of Camellia yuhsienensis Hu
作  者：
PENG Qiu-fa et al (College of Chemistry and Life Science, Zhejiang Normal University, Jinhua, Zhejiang 321004)
关键词：
Camellia yuhsienensis Hu; Somatic embryo; In vitro; Histological observations
摘  要：
[Objective] The research aimed to investigate the somatic embryogenesis from Camellia yuhsienensis Hu using cotyledons as explants. [Method] Embryogenic calluses were obtained from cotyledons which had been cultured in MS+2.0 mg/L 2, 4-D+1.0 mg/L KT. Then, embryogenic calluses were incubated for somatic embryogenesis in MS basic medium with different concentrations of 6-BA and NAA. Somatic embryos at different developmental stages were sectioned and observed under light microscope. [Result] The results showed that the embryogenic calluses were initiated within 15 d. The optimal medium for somatic embryo differentiation was MS + 2.0 mg/L 6-BA + 0.6 mg/L NAA and the induction frequency was 87.78%. The somatic embryos were also formed in the MS medium without phytohormones, but the induction frequency was less than 6.67%. The histological observations indicated that somatic embryos originated from epidermal cells or some cells near epidermis. The mature somatic embryos were finally formed via globular embryo, heart-shaped embryo and cotyledon-shaped embryo stages. [Conclusion] The optimal medium for somatic embryos differentiation from embryogenic callues was MS +2.0 mg/L 6-BA + 0.6 mg/L NAA.

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