当前位置: 首页 > 文章 > 黑斑蛙皮肤抗菌肽的纯化及抑菌活性研究 黑龙江畜牧兽医 2014 (11) 167-168+172
Position: Home > Articles > Study on purification and antibacterial activity of antimicrobial peptides from the skin of Rana nigromaculata Heilongjiang Animal Science and Veterinary Medicine 2014 (11) 167-168+172

黑斑蛙皮肤抗菌肽的纯化及抑菌活性研究

作  者:
王雅丽[1;李吉平;郝镯;李楠;李树民
单  位:
吉林农业大学动物科技学院军事医学科学院军事兽医研究所沈阳市畜牧兽医科学所;吉林农业大学;军事医学科学院军事兽医研究所
关键词:
黑斑蛙;抗菌肽(AMPs);纯化;抑菌活性
摘  要:
为了从黑斑蛙皮肤中获得纯度高且抑菌活性较强的抗菌肽(AMPs),试验采用稳压电刺激的方法提取黑斑蛙皮肤分泌物的粗提物。结果表明:黑斑蛙皮肤分泌物的粗提物对大肠杆菌和金黄色葡萄球菌均有抑菌活性。利用高效液相色谱法对其进行纯化,通过纯化获得了一种纯度高且抑菌活性较强的AMPs,经过质谱鉴定,这种AMPs的相对分子质量为2 878.102。
译  名:
Study on purification and antibacterial activity of antimicrobial peptides from the skin of Rana nigromaculata
作  者:
WANG Ya-li;LI Ji-ping;HAO Zhuo;LI Nan;CAI Yu-feng;LI Shu-min;LIU Quan;WEI Feng;Jilin Agricultural University;Institute of Military Veterinary,Academy of Military Medical Sciences;
关键词:
Rana nigromaculata;;antimicrobial peptide(AMPs);;purification;;antibacterial activity
摘  要:
To obtain antimicrobial peptides( AMPs) with high purity and strong antibacterial activity from the skin of Rana nigromaculata,the method of regulated-voltage electrical stimulation was used to extract the crude extract of skin secretion from Rana nigromaculata. The results showed that the crude extract of skin secretion from Rana nigromaculata had antibacterial activity against Escherichia coli and Staphylococcus aureus. The high-performance liquid chromatography was used to purify the crude extract,and then one kind of AMPs with high purity and strong antibacterial activity was obtained. It had a relative molecular weight of 2 878. 102,which was identified by mass spectrometry.

相似文章

计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊