当前位置: 首页 > 文章 > 小鼠卵母细胞去核方法的初步研究 安徽农业科学 2007,35 (32) 10305-10306
Position: Home > Articles > Preliminary Study on the Enucleation Method of Mouse Oocyte Journal of Anhui Agricultural Sciences 2007,35 (32) 10305-10306

小鼠卵母细胞去核方法的初步研究

作  者:
淡新刚;史远刚
单  位:
宁夏大学农学院
关键词:
卵母细胞;去核;负压-点压法
摘  要:
[目的]寻求既简单又有效的提高小鼠卵母细胞去核率的方法。[方法]分别采用3种不同的方法(盲吸法、点击法、负压-点压法),研究对MII期小鼠卵母细胞去核率的影响。[结果]结果表明,采用负压-点压法去核,其卵母细胞的去核率可达62.8%,与其他两种方法所获得的去核率差异显著(P<0.05)。[结论]负压-点压法是一种简单、有效的小鼠卵母细胞去核方法。
译  名:
Preliminary Study on the Enucleation Method of Mouse Oocyte
作  者:
DAN Xin-gang et al(College of Agriculture,Ningxia University,Yinchuan,Ningxia 750021)
关键词:
Oocytes;Enucleation methods;Negative-pressing point-pressing method
摘  要:
[Objective] This experiment is to explore simple and effective method to improve mouse oocyte enucleation rate.[Method] Mouse MII oocytes were enucleated by three methods: Blind-sucking,point-hitting and negative-pressing and point-pressing.[Result] The rate of enucleation was 62.8 % by negative-pressing and point-pressing,and the difference were significant as compared with other groups(P<0.05).[Conclusion] negative-pressing point-pressing method is a simple and effective method for mouse oocyte enucleation.

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