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当前位置: 首页 > 文章 > 基于关联分析发掘烟草高钾优异等位变异 中国烟草学报 2016,22 (2) 100-107
Position: Home > Articles > Exploring high-potassium favorable allele mutation of tobacco based on genome-wide association analysis Acta Tabacaria Sinica 2016,22 (2) 100-107

基于关联分析发掘烟草高钾优异等位变异

作  者：
樊文强;孙鑫;杨爱国;程立锐;张忠锋;任民
单  位：
中国农业科学院烟草研究所
关键词：
烟草;高钾;关联分析;SSR标记;优异等位变异
摘  要：
采用全基因组454个SSR位点对96份烤烟种质资源进行了群体分型,获得有效等位变异1038个。NJ聚类分析将供试材料分为3个类群,群体结构分析表明,当K=3时,ΔK值最大。同时对该供试材料在4个环境进行了烟叶钾含量测定,其频率符合正态分布。采用混合线性模型(MLM),进行了标记-性状关联分析,获得11个烟草钾含量的关联标记。通过不同基因型的钾含量对比,获得5个高钾优异等位变异。为验证这些优异等位变异,本研究又利用这5个关联标记扫描了其他130份烟草种质,获得了一致的结果。表明可以利用这5个高钾等位变异对烟草种质资源进行定性筛选,促进烟草高钾种质的利用和品种选育。
译  名：
Exploring high-potassium favorable allele mutation of tobacco based on genome-wide association analysis
作  者：
FAN Wenqiang;SUN Xin;YANG Aiguo;CHENG Lirui;ZHANG Zhongfeng;REN Min;Key Laboratory of Tobacco Genetic Improvement and Biotechnology, Tobacco Research Institute, Chinese Academy of Agricultural Sciences;Postgraduate School, Chinese Academy of Agricultural Sciences;
关键词：
tobacco;;high-potassium;;association analysis;;SSR marker;;favorable allele mutation
摘  要：
1038 effective alleles were obtained by screening 96 tobacco germplasm based on 454 polymorphic SSR loci.Neighbor-Joining cluster analysis showed that there existed 3 groups in 96 lines,of which AK was highest when K was equal to 3.Potassium content of the96 lines was simultaneously tested in 4 cultivated environments and its frequency was in line with normal distribution.11 SSR markers associated with potassium content in tobacco leaves were gained through genome-wide association analysis based on mixed liner model.5high-potassium alleles were developed by comparing potassium content in different alleles.Another 130 tobacco lines were screened with the 5 associated SSR markers to have further vindicated the feasibility of using the 5 SSR markers to screen high-potassium germplasm and thus to improve the quality of tobacco breeding and selection.

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