Cai YuMei;Li QingLan;Lin MaoFan;Shangrao P

Dujiangyan College of Sichuan Agricultural University,Dujiangyan,Sichuan 611830,Chin;Dujiangyan College of Sichuan Agricultural University,Dujiangyan,Sichuan 611830,China

taxus media;root;different ages;taxol content;00;reliabl

[Objective]To develop a reliable HPLC method for determining the dynamic change of taxol content in the root of Taxus media at different ages.[Method]The HPLC conditions were as follows:Phenomenex Luna C18 column(250 mm×4.6 mm,5 μm),mobile phase of methanol-acetonitrile-water,flow rate of 1.0 ml/min,detection wavelength of 227 nm,column temperature of 40 ℃ and injecting amount of 10 μl.[Result]The taxol was well separated under the above conditions.The linear relationship was good in the range of 1.00-32.00 mg/L(r=0.999 9),the average recovery rate and RSD values were 100.3% and 1.6% respectively.The content of taxol in the root of Taxus media at different ages was varied.[Conclusion]A reliable and convenient method was firstly established to determine the taxol content in the root of Taxus media.