当前位置: 首页 > 文章 > 真菌细胞壁抑制剂刚果红诱导酵母细胞凋亡及其机制研究 河南农业科学 2015,44 (12) 65-69
Position: Home > Articles > Fungal Cell Wall-perturbing Agent Congo Red-induced Yeast Apoptosis and Its Underlying Mechanisms Journal of Henan Agricultural Sciences 2015,44 (12) 65-69

真菌细胞壁抑制剂刚果红诱导酵母细胞凋亡及其机制研究

作  者:
张小华;孙业盈;卞伟华;许聪;武玉永;刘向勇
单  位:
滨州医学院药学院
关键词:
细胞壁;刚果红;酿酒酵母;细胞凋亡;细胞壁抑制剂
摘  要:
以酿酒酵母为研究材料,分析真菌细胞壁抑制剂刚果红(CR)对酵母细胞凋亡的影响及其机制,为植物病原真菌细胞凋亡机制研究提供新的理论依据。结果表明:与正常对照组相比,CR处理后酵母细胞存活率下降、活性氧升高、磷脂酰丝氨酸外翻、细胞核裂解,发生细胞凋亡;外源添加抗氧化剂N-乙酰半胱氨酸(NAC)、过表达超氧化物歧化酶基因SOD1和缺失YCA1基因,均可以显著降低CR诱导的酵母细胞凋亡。以上结果说明,CR可以诱导ROS和Yca1p依赖的酵母细胞凋亡。
译  名:
Fungal Cell Wall-perturbing Agent Congo Red-induced Yeast Apoptosis and Its Underlying Mechanisms
作  者:
ZHANG Xiaohua;SUN Yeying;BIAN Weihua;XU Cong;WU Yuyong;LIU Xiangyong;Department of Pharmacy,Binzhou Medical College;
关键词:
cell wall;;Congo red;;Saccharomyces cerevisiae;;apoptosis;;cell wall-perturbing agents
摘  要:
This paper investigated the effect of fungal cell wall-perturbing agent Congo red( CR) treatment on apoptosis of Saccharomyces cerevisiae and its underlying mechanisms,to provide a new theoretical foundation for study of plant pathogenic fungi apoptosis in future. The results showed that the CR-treated yeast cells displayed more serious apoptosis compared with the normal control group,including decrease of cell survival rate,increase of reactive oxygen species,phosphatidylserine externalization,and nuclei lysis.The CR-induced yeast apoptosis was suppressed by addition of antioxidant N-acetyl-L-cysteine( NAC),overexpression of Cu,Zn-superoxide dismutase encoding gene SOD1 or deletion of YCA1 gene. Taken together,CR could induce ROS and Yca1p-dependent yeast apoptosis.

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