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当前位置: 首页 > 文章 > 黄瓜、甜瓜和西瓜MLO基因家族的比较基因组学分析 核农学报 2014,28 (6) 1006-1017
Position: Home > Articles > Comparative Genomics Analysis of MLO Gene Family in Cucumber,Melon and Watermelon Journal of Nuclear Agricultural Sciences 2014,28 (6) 1006-1017

黄瓜、甜瓜和西瓜MLO基因家族的比较基因组学分析

作  者：
徐坚;陈先知;王燕;史建磊;朱隆静;王克磊
单  位：
温州科技职业学院;温州市农科院蔬菜科学研究所/浙南作物遗传育种重点实验室
关键词：
黄瓜;甜瓜;西瓜;MLO;比较基因组分析
摘  要：
MLO基因是植物特有的一类基因家族,在调控植物抵抗生物和非生物胁迫方面起着重要作用。为了揭示葫芦科作物MLO基因的遗传变异及系统发育关系,本文以黄瓜、甜瓜和西瓜基因组数据为基础,利用生物信息学方法对其MLO基因家族进行鉴定与分析。结果发现,黄瓜、甜瓜和西瓜基因组中共含有42个MLO基因家族成员,每一个物种均含有14个成员,且保守性强;系统发育关系揭示了这些成员在黄瓜、甜瓜和西瓜基因组中并不呈现一一对应关系,表明MLO型基因在这3种植物分化之后分别发生了扩展和丢失;进一步将其与模式植物拟南芥、番茄、豌豆MLO型白粉病基因进行聚类分析:一方面,借助于拟南芥MLO基因的分类标准,揭示了在黄瓜、甜瓜和西瓜基因组中也存在双子叶植物MLO型白粉病基因的特异区组,他们各自至少包含3个候选的白粉病基因,序列比对进一步发现这些基因均具有MLO型白粉病基因的典型结构特征,如7个跨膜结构域、钙调蛋白结合区以及两个缩氨酸区域(I和II);另一方面,大部分区组中包含的MLO基因均来源于拟南芥和黄瓜、甜瓜和西瓜MLO基因家族的成员,表明了MLO基因家族在拟南芥和葫芦科作物分化之前就已经存在。EST表达分析表明MLO基因广泛地参与到黄瓜、甜瓜和西瓜器官的营养生长和生殖生长。研究结果为揭示黄瓜、甜瓜和西瓜MLO基因的进化关系、功能及克隆表达分析奠定了基础。
译  名：
Comparative Genomics Analysis of MLO Gene Family in Cucumber,Melon and Watermelon
作  者：
XU Jian;CHEN Xian-Zhi;WANG Yan;SHI Jian-Lei;ZHU Long-Jing;WANG Ke-Lei;Key Laboratory of Crop Breeding in South Zhejiang/Institute of Vegetable Crops,Wenzhou Academy of Agricultural Sciences,Wenzhou Vocation College of Science & Technology;
关键词：
Cucumber;;Melon;;Watermelon;;MLO;;Comparative genomics analysis
摘  要：
MLO is a plant-peculiar gene family,and plays an important role in plant response to powdery mildew infection. To elaborate genetic diversity and phylogenetic relationship of MLO genes in Curcurbitaceae crops,three crop genome data( cucumber,melon and watermelon) were used to identify MLO gene family of members through bioinformatics method. Results show that a total of 42 MLO genes were identified in cucumber( 14),melon( 14) and watermelon( 14),respectively. Phylogenetic relationship reveals that the members of these gene family in cucumber,melon and watermelon is not present corresponding relationship( 1: 1: 1),which showed that gene expand and loss have occurred after differentiation of three plant species. Furthermore,Phylogenetic tree of MLO genes from the model plant Arabidopsis thaliana,tomato,pea and cucumber,melon,and watermelon was constructed. On the one hand,based on the classification criterion in Arabidopsis,a dicot-specific susceptible to powdery mildew group was identified. There are at least three candidate genes related to powdery mildew in the cucumber,melon and watermelon genome,and these genes have typical of structure characteristics of MLO type of powdery mildew disease genes, such as seventransmembrane domains,calmodulin-binding protein domain and peptide domains( I and II). On the other hand,most of these clades were comprised of MLO genes from A. thaliana,cucumber,melon,and watermelon,suggesting that these genes may have existed before differentiation of Arabidopsis and Cucurbitaceae crops. EST-based expression analysis showed that MLO gene is involved widely in vegetative and reproductive growth in cucumber,melon and watermelon. These results will lay a foundation for understanding original,function,clone and expression analysis of MLO genes in cucumber,melon and watermelon in the future.

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