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当前位置: 首页 > 文章 > 捕食线虫性真菌Duddingtonia flagrans冻干制剂的杀虫效果 中国兽医学报 2017 (12) 2310-2314
Position: Home > Articles > Nematodelarvae disinfestations treated with lyophilized preparation of nematodetrapping fungus-Duddingtonia flagrans Chinese Journal of Veterinary Science 2017 (12) 2310-2314

捕食线虫性真菌Duddingtonia flagrans冻干制剂的杀虫效果

作  者：
李莹莹;乔蕾;杨晓野;王瑞;陈林军;李军燕;罗晓平;连建华
单  位：
内蒙古自治区农牧业科学院兽医研究所;内蒙古农业大学兽医学院农业部动物疾病临床诊疗技术重点实验室;内蒙古自治区额尔古纳市动物疫病预防与控制中心
关键词：
Duddingtonia flagrans;冻干制剂;杀虫效果
摘  要：
本试验通过在含有0.05%琼脂粉的沙氏葡萄糖肉汤培养基中培养Duddingtonia flagrans(D.flagrans)再转接到玉米粒培养基的方法,对D.flagrans厚壁孢子进行培养,之后将其洗脱并制备成冻干制剂,然后对冻干制剂的萌发率和杀虫率进行观察。结果显示,D.flagrans冻干制剂厚壁孢子在接种玉米粉琼脂培养基(CMA)培养后5d萌发率达到峰值78.3%;经口饲喂绵羊D.flagrans冻干厚壁孢子剂量为1×106个/kg时,幼虫平均减少率为81.4%。结果表明,D.flagrans冻干制剂使用方便,复苏和杀虫效果较好,其在生物防制家畜胃肠道线虫病方面有很大的应用前景。
译  名：
Nematodelarvae disinfestations treated with lyophilized preparation of nematodetrapping fungus-Duddingtonia flagrans
作  者：
LI Ying-ying;QIAO Lei;YANG Xiao-Ye;WANG Rui;CHEN Lin-jun;LI Jun-yan;LUO Xiao-ping;LIAN Jian-hua;Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease,College of Veterinary Medicine,Inner Mongolia Agricultural University;Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences;Center for Animal Disease Control and Prevention,Eerguna City of Hulunbeir;
关键词：
Duddingtonia flagrans;;lyophilized preparation;;effect on kill nematodes
摘  要：
In order to obtain the germination rate and insecticidal effect of freeze-dried nematodetrapping fungus-Duddingtonia flagrans(D.flagrans),the fungus first incubated in sabourauddextrose broth medium with 0.05%agar,and then transferred to the corn medium.After cultured,the fungi were chlamydospores from media eluted and lyophilized.The results showed thatthe maximum germination rate of lyophilized D.flagrans chlamydospores was 78.3% on the 5 th day after inoculation;the average percent reduction of nematodes larves was 81.4% when the sheep were feed by freeze-dry preparation at the dose of 106 fungal spores/kg body weight.The results indicated that the freeze-dried D.flagrans production is not only convenient to use but also has good effect on recovering and killing nematode.Proved by the trials,this lyophilized preparation of D.flagrans has great application prospects in biological control of livestock gastrointestinal nematodes.

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