作 者：
邢月腾;郁元年;谢春艳;吴信;肖定福;杨志武;张彬
摘 要：
本试验旨在研究亚麻油对宁乡猪背肌和3种不同脂肪组织中长链脂肪酸谱的影响,并探究脂肪酸生成的潜在机制。选取日龄相近、平均体重(43.21±0.64) kg的宁乡阉母猪30头,随机分为2组,每组5个重复,每个重复3头。对照组饲喂基础饲粮,试验组在基础饲粮中添加2%亚麻油,试验期56 d。结果显示:与对照组相比,饲粮中添加2%亚麻油后,宁乡猪背肌中花生一烯酸(C20∶1)、γ-亚麻酸(C18∶3n-6)、α-亚麻酸(C18∶3n-3)含量显著提高(P<0.05),花生四烯酸(C20∶4n-6)含量显著降低(P<0.05);饲粮中添加2%亚麻油对3个脂肪组织中脂肪酸含量的影响与背肌趋于一致,如能显著提高背脂、腹脂和肾周脂中亚油酸(C18∶2n-6c)、C18∶3n-6、C18∶3n-3、二十碳三烯酸(C20∶3n-6)含量(P<0.05),降低背脂和腹脂中油酸(C18∶1n-9c)(P<0.05)、C20∶4n-6(0.05≤P<0.10)及肾周脂中硬脂酸(C18∶0)含量(P<0.05);同时,饲粮中添加2%亚麻油还能显著降低3种脂肪组织中n-6/n-3多不饱和脂肪酸(PUFA)比值(P<0.05),使3种脂肪组织中平均n-6/n-3 PUFA比值降到5.92。此外,饲粮中添加2%亚麻油能显著下调背脂中乙酰辅酶A羧化酶α(ACCα)、γ-过氧化酶活化增生受体(PPARγ)和脂肪酸去饱和酶2(FADS2)基因的表达(P<0.05),对α-过氧化酶活化增生受体(PPARα)基因的表达也有抑制趋势(0.05≤P<0.10)。上述结果表明,饲粮中添加2%亚麻油能显著提高宁乡猪背肌、背脂、腹脂和肾周脂中C18∶3n-3含量,使3种脂肪组织中平均n-6/n-3 PUFA比值降至《中国居民膳食指南(2016)》推荐范围(4～6)内;这可能是通过调控细胞分化及抑制脂肪酸从头合成途径实现的。
译 名：
Effects of Linseed Oil on Long-Chain Fatty Acid Profile in Different Tissues of Ningxiang Pigs
作 者：
XING Yueteng;YU Yuannian;XIE Chunyan;WU Xin;XIAO Dingfu;YANG Zhiwu;ZHANG Bin;College of Animal Science and Technology,Hunan Agricultural University;College of Resources and Environment,Hunan Agricultural University;Institute of Subtropical Agriculture,Chinese Academy of Sciences;Ningxiang Animal Husbandry and Veterinary Bureau;
关键词：
linseed oil;;long-chain fatty acid;;lipid metabolism;;Ningxiang pigs
摘 要：
This experiment was conducted to study the linseed oil(LO)on fatty acid profile in longissimus dorsi muscle(LDM)and three different adipose tissues of Ningxiang pigs,and then investigate the underlying lipogenesis mechanisms. Thirty castrated female pigs with a similar age and average body weight of(43.21±0.64)kg were randomly allocated into 2 groups with 5 repetitions per group and 3 pigs per repetition. Pigs in control group were fed a basal diet,and those in experimental group were fed the basal diet supplemented with2% LO for 56 days. The results showed that dietary supplemented with 2% LO significantly increased the contents of eicosenoic acid(C20∶1), γ-linolenic acid(C18∶3 n-6)andα-linolenic acid(C18∶3 n-3)in LDM(P<0.05),and significantly decreased the content of arachidonic acid(C20∶4 n-6)in LDM compared with control group(P<0.05). The fatty acid contents in 3 adipose tissues influenced by 2% LO were analogous to those found in the LDM. The contents of linoleic acid(C18∶2 n-6 c),C18∶3 n-6,C18∶3 n-3 and epoxyeicosatrienoic acid(C20∶3 n-6)in dorsal subcutaneous adipose(DSA),abdominal subcutaneous adipose(ASA)and perirenal adipose(PA)were significantly increased(P<0.05)accompanied by a corresponding decrease in oleic acid(C18∶1 n-9 c)(P<0.05),C20∶4 n-6(0.05≤P<0.10)in DSA and APA,as well as stearic acid(C18∶0)in PA(P<0.05). Meanwhile,dietary supplemented with 2% LO could significantly the ratio of n-6/n-3 polyunsaturated fatty acids(PUFA)in 3 adipose tissues(P<0.05),and make the average n-6/n-3 PUFA ratio reduce to 5.92. Furthermore,dietary supplemented with 2% LO could down-regulate the expression of acetyl coenzyme A carboxylaseα (ACCα),peroxisome proliferators-activated receptorγ (PPARγ),fatty acid desaturase 2(FADS2)genes in DSA(P<0.05),and had an inhibitory tendency on the expression of peroxisome proliferators-activated receptorα (PPARα)gene in DSA(0.05≤P<0.10). These results indicate that dietary supplemented with 2% LO can significantly increase the content of C18∶3 n-3 in LDM,DSA,ASA and PA of Ningxiang pigs,and make the average n-6/n-3 PUFA ratio in three adipose tissues down to 5.92,which in the recommended range(4 to 6)of Dietary Guidelines for Chinese Residents(2016). The regulation of LO on lipid metabolism may be achieved through regulating the de novo synthesis of fatty acids and cell differentiation.
