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当前位置: 首页 > 文章 > 黄瓜枯萎病拮抗放线菌筛选及其生防作用鉴定 园艺学报 2012,39 (6) 1123-1130
Position: Home > Articles > Isolation and Identification of Antagonistic Actinomycetes and Their Application in the Biocontrol of Fusarium oxysporum f. sp. cucumerinum Acta Horticulturae Sinica 2012,39 (6) 1123-1130

黄瓜枯萎病拮抗放线菌筛选及其生防作用鉴定

作  者：
刘金秀;马正;申屠旭萍;于冰;俞晓平
单  位：
浙江省生物计量及检验检疫技术重点实验室;中国计量学院生命科学学院
关键词：
黄瓜;枯萎病;拮抗放线菌;菌株鉴定;生防作用;毒三素链霉菌
摘  要：
通过高效筛选法从不同环境采集的土样中分离出44株对黄瓜枯萎病菌有拮抗作用的菌株,通过平板对峙法初筛和复筛得到一株具有较高拮抗活性的放线菌XF-7。研究了菌株XF-7发酵液对黄瓜枯萎病病原菌孢子萌发、菌丝生长及黄瓜种子发芽的影响,以及对苗期枯萎病的生防效果。结果表明:菌株XF-7发酵液对黄瓜枯萎病菌的菌丝生长及孢子萌发均有明显的抑制作用,可促进黄瓜种子发芽,且对黄瓜苗期枯萎病的防治效果显著,防效达79.57%。经16S rDNA序列分析,发现菌株XF-7与毒三素链霉菌(Streptomyces toxytricini)的16S rDNA序列同源性达100%。结合形态特征和生理生化特性,将菌株XF-7鉴定并命名为Streptomyces toxytricini XF-7,在GenBank中该菌株的序列登录号为JN315670。
译  名：
Isolation and Identification of Antagonistic Actinomycetes and Their Application in the Biocontrol of Fusarium oxysporum f. sp. cucumerinum
作  者：
LIU Jin-xiu,MA Zheng,SHENTU Xu-ping,YU Bing,and YU Xiao-ping*(College of Life Sciences,China Jiliang University,Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine,Hangzhou 310018,China)
关键词：
cucumber;Fusarium oxysporum f.sp.cucumerinum;antagonistic actinomycetes;identification;biocontrol;Streptomyces toxytricini
摘  要：
To obtain antagonistic actinomycetes against plant pathogen,Fusarium oxysporum f.sp.cucumerinum,about 44 strains of actinomycetes with antagonistic activity were isolated from various soil samples.Through the primary and secondary screening,the strain XF-7 showed the most efficient antagonistic activity among all tested strains.Moreover,the effects of fermentation broth of strain XF-7 on mycelium growth,spore germination of F.oxysporum f.sp.cucumerinum,seed germination of cucumber were tested,and the clay-pot experiments on the biocontrol effects of fermentation broth on seedling fusarium wilt were conducted.The results indicated that the fermented broth of strain XF-7 had a significant inhibition on the mycelium growth and spore germination of plant pathogen,and had a stimulative effect on seed germination of cucumber,the control efficacy against seedling fusarium wilt of cucumber was significantly up to 79.57% as compared to the control.The 16S rDNA sequence of strain XF-7 shared 100% homology with Streptomyces toxytricini,strain XF-7 was morphologically and molecularly identified and named as S.toxytricini XF-7,with GenBank accession number JN315670.

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