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Position: Home > Articles > Studies on RAPD Marker of Bacterial Wilt Resistance Gene in Eggplant(Solanum melongena) Acta Horticulturae Sinica 2005,32 (2) 321-323

茄子抗青枯病基因的RAPD标记研究

作  者:
朱华武;姚元干;刘志敏;杨建国;陈惠明;邹学校
单  位:
湖南省蔬菜研究所;湖南农业大学园艺园林学院
关键词:
茄子;抗青枯病基因;分子标记
摘  要:
为探明茄子抗青枯病的遗传机制,以高感青枯病品种北京六叶茄064,高抗青枯病半栽培种马来西亚S3及其F2代为材料,用RAPD技术对供试材料的抗青枯病基因进行了研究。结果表明:通过300个随机引物的PCR扩增分析,发现15.6%的引物在双亲间表现出多态性,找到了一个与茄子抗青枯病亲本S3中的抗病基因紧密连锁的分子标记S264780(该引物的碱基序列为CAGAGCGGA),该标记与S3的抗病基因的交换值为4.32%,遗传距离为4.33cM。
译  名:
Studies on RAPD Marker of Bacterial Wilt Resistance Gene in Eggplant(Solanum melongena)
作  者:
Zhu Huawu1, Yao Yuangan2,Liu Zhimin1, Yang Jianguo2, Chen Huiming2, and Zou Xuexiao2 ( 1College of Horticulture and Gardening,Hunan Agricultural University, Changsha 410128,China; 2Hunan Vegetable Research Institute, Changsha 410125,China)
关键词:
Solanum melongena;Bacterial wilt resistance gene; RAPD
摘  要:
To probe into the genetic resistant law to bacterial wilt(BW)in eggplant,the genetic studies to BW of 064(Beijing six-leaf eggplant),S3( highly resistant half-cultivated species of Malasia) and 064×S3 F_2 populations is carried out on RAPD marker.300 decamer primers with arbitrary sequences are chosen for polymerase chain reaction amplication.It is found that the polymorphism between the parents could be detected by 15.6% of the primer; one RAPD marker S264_ 780 (the sequence of the primer:CAGAAGCGGA) tightly links to the resistance gene of S3 with the overcross value of 4.32% and genetic distance of 4.33 cM.

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