Position: Home > Articles > Cloning and sequence analysis of Paralichthys olivaceus Follistatin cDNA.
Journal of Zhejiang University (Agriculture and Life Sciences)
2007,33
(6)
621-625
牙鲆Follistatin cDNA的克隆与序列分析
作 者：
刘庆华;谭训刚;徐永立;张培军;徐芃
单 位：
中国科学院海洋研究所实验海洋生物学重点实验室
关键词：
牙鲆(Paralichthysolivaceus);卵泡抑素(Follistatin);序列分析
摘 要：
为研究海水鱼中Follistatin基因的序列特征,从受精后67 h的牙鲆受精卵中克隆获得牙鲆Follistatin基因cDNA序列,并对其进行序列分析.结果表明,在牙鲆中并没有发现Follistatin基因的可变式剪切现象;牙鲆Follistatin基因cDNA序列全长963 bp.序列分析结果显示:牙鲆与Takifugurubripes的亲缘关系最近.Follistatin蛋白中高度保守的半胱氨酸和甘氨酸残基的生物学功能可能是维持蛋白空间结构的稳定.
译 名：
Cloning and sequence analysis of Paralichthys olivaceus Follistatin cDNA.
作 者：
LIU Qing-hua1,2,TAN Xun-gang1,XU Yong-li1,ZHANG Pei-jun1,XU Peng1 (1.Key Laboratory of Experimental Marine Biology,Institute of Oceanology,Chinese Academy of Sciences,Qingdao,Shandong 266071,China;2.Graduate School,Chinese Academy of Sciences,Beijing 100039,China)
关键词：
Paralichthys olivaceus;Follistatin;sequence analysis
摘 要：
To study the DNA sequence features of Follistatin gene in marine fish, Paralichthys olivaceus Follistatin cDNA was isolated and analyzed from Paralichthys olivaceus embryos 67 h after fertilization.The results indicated that there was no evidence for alternative splicing of Follistain in Paralichthys olivaceus.The length of Paralichthys olivaceus Follistatin cDNA was 963 bp.Sequence analysis revealed that Paralichthys olivaceus Follistatin was more closely related to Takifugu rubripes.The biological function of the highly conserved cysteine and glycin in Paralichthys olivaceus Follistatin protein might be maintaining the stabilization of protein structure.
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