作 者：
邝良德;雷岷;李丛艳;任永军;郭志强;郑洁;张翔宇;张翠霞;杨超;唐丽;邓小东;谢晓红
单 位：
四川省畜牧科学研究院动物遗传育种四川省重点实验室
关键词：
齐兴肉兔;肌肉;转录组测序;生长发育;差异表达基因
摘 要：
为了研究不同发育阶段齐兴肉兔肌肉生长发育相关的差异表达基因,试验选取胚胎期18日龄、26日龄的齐兴肉兔骨骼肌进行转录组测序,与兔基因组数据库进行比对,筛选差异表达基因并进行GO分析,进一步筛选与肌肉生长发育相关的差异表达基因,并通过qRT-PCR对筛选基因进行验证.结果表明:胚胎期26日龄共获得5 320个差异表达基因,其中上调基因2 607个、下调基因2 713个;KEGG功能注释发现差异基因显著富集到癌症通路、细胞骨架调控通路、pi3k-akt信号通路和RAP1信号通路;筛选获得的6个基因与肌肉生长发育关系较为密切,其中上调基因分别为TPM1、CASQ1、MYOZ1、CKM、MYL3基因,下调基因为IGF2BP1基因.
作 者：
KUANG Liangde;LEI Min;LI Congyan;REN Yongjun;GUO Zhiqiang;ZHENG Jie;ZHANG Xiangyu;ZHANG Cuixia;YANG Chao;TANG Li;DENG Xiaodong;XIE Xiaohong;Animal Breeding and Genetics Key Laboratory of Sichuan Province,Sichuan Animal Science Academy;
单 位：
KUANG Liangde%LEI Min%LI Congyan%REN Yongjun%GUO Zhiqiang%ZHENG Jie%ZHANG Xiangyu%ZHANG Cuixia%YANG Chao%TANG Li%DENG Xiaodong%XIE Xiaohong%Animal Breeding and Genetics Key Laboratory of Sichuan Province,Sichuan Animal Science Academy
关键词：
Qixing rabbit;;muscle;;transcriptome sequencing;;growth and development;;differential expression gene
摘 要：
In order to study the differential expression genes related to muscle growth and development in different developmental stages of Qixing rabbits,the Qixing rabbits of 18 and 26 days of the embryonic age were selected in this experiment for skeletal muscle transcriptome sequencing.Compared with the rabbit genome database,the differential expression genes were screened,and the GO analysis was carried out for the further screening of the differential expression genes related to muscle growth and development and qRT-PCR was performed for the validation of the selected genes. The results showed that 5 320 differential expression genes were obtained between 26 days and 18 days of the embryonic age,which contained 2 607 up-regulated genes and 2 713 down-regulated genes. KEGG functional annotation revealed that the differential expression genes were significantly enriched in cancer pathway,cytoskeleton regulatory pathway,pi3 k-akt signaling pathway and RAP1 signaling pathway.Six screened genes were obtained,which were closely related to muscle growth and development. Among them,the up-regulated genes were TPM1,CASQ1,MYOZ1,CKM and MYL3,the down-regulated gene was IGF2 BP1.
