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Position: Home > Articles > Immunoscreening of cDNA expression library of Haemaphysalis qinghaiensis adult tick and analysis of positive clones Chinese Veterinary Science 2008,38 (9) 743-746

青海血蜱成蜱cDNA表达文库的免疫学筛选及阳性克隆分析

作  者:
李金杰;李有全;刘军龙;赵海平;高金亮;殷宏;罗建勋
单  位:
中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室
关键词:
青海血蜱;cDNA表达文库;免疫学筛选
摘  要:
采用兔抗青海血蜱幼蜱阳性血清,对青海血蜱成蜱cDNA表达文库进行免疫学筛选,并对获得的阳性克隆进行序列测定及同源性分析。结果显示,共获得9个新cDNA序列,其中3个新基因序列已被GenBank接受,并获得序列接受号。表明,从青海血蜱成蜱cDNA表达文库筛选到一批新基因,为青海血蜱保护性抗原的筛选奠定了基础。
译  名:
Immunoscreening of cDNA expression library of Haemaphysalis qinghaiensis adult tick and analysis of positive clones
作  者:
LI Jin-jie,LI You-quan,LIU Jun-long,ZHAO Hai-ping,GAO Jin-liang,YIN Hong,LUO Jian-xun(Key Laboratory of Veterinary Parasitology of Gansu Province/State Key Laboratory of Veterinary Etiological Biology/Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China)
关键词:
Haemaphysalis qinghaiensis;cDNA expression library;immunoscreening
摘  要:
cDNA expression library of Haemaphysalis qinghaiensis adult tick was immunoscreened with positive serum of rabbit against H.qinghaiensis larval tick.The obtained positive clones were sequenced and analyzed.Nine novel genes were found,and the accession numbers for three of them were obtained from GenBankTM.The result revealed that some novel genes were immunoscreened successfully,providing basis for the screening of protective antigens of H.qinghaiensis .

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