中国农业期刊集成服务平台

当前位置: 首页 > 文章 > 4个群体鲢mtDNA D-loop的PCR-RFLP分析淡水渔业 2010,40 (4) 5-11+17

Position: Home > Articles > PCR-RFLP analysis on mtDNA D-loop region of four populations of silver carp (Hypophthalmichthys molitrix) Freshwater Fisheries 2010,40 (4) 5-11+17

4个群体鲢mtDNA D-loop的PCR-RFLP分析

作者：

王淞;曹晓霞;谷口顺彦;董仕

单位：

福山大学生命工学部;天津师范大学生命科学学院

关键词：

鲢(Hypophthalmichthys molitrix);mtDNAD-loop;PCR-RFLP;遗传多样性;Rogers遗传距

摘要：

采用PCR技术扩增了湖北监利、江西瑞昌、湖南长沙3个长江野生群体和天津宁河1个人工繁殖群体(已繁殖6代)共158尾鲢(Hypophthalmichthys molitrix)mtDNA D-loop区段,并用14种核酸内切限制酶对扩增片段进行酶切。结果显示:所有试验鱼均扩增出约1.6 kb的DNA片段,10种限制酶有酶切位点,共检测出16种单倍型,以单倍型Ⅰ为主,在各群体中所占比例为57.5%~90.0%。鲢4个群体的单倍型多样性指数、核苷酸多样性指数为0.1885~0.6526和0.001182~0.007570。瑞昌群体与监利群体的Rogers遗传距最小,为0.1250;监利群体与宁河群体的Rogers遗传距最大,为0.2693。χ2检验结果显示4个群体间的遗传差异显著(P<0.01)。在4个群体中,宁河人工繁殖群体的单倍型多样性指数、核苷酸多样性指数均最高,并且HinfⅠ的C酶切类型为宁河群体所特有,达20.0%。

译名：

PCR-RFLP analysis on mtDNA D-loop region of four populations of silver carp (Hypophthalmichthys molitrix)

作者：

WANG Song1,CAO Xiao-xia1,TANIGUCHI Nobuhiko2,DONG Shi1(1.College of Life Science,Tianjin Key Laboratory of Cyto-Genetical and Molecular Regulation,Tianjin Normal University,Tianjin 300387;2.Faculty of Life Science and Biotechnology,Fukuyama University,Fukuyama,Japan 722-2101)

关键词：

silver carp(Hypophthalmichthys molitrix);mtDNA D-loop;PCR-RFLP;genetic diversity;Rogers′ genetic distance

摘要：

The restriction fragment length polymorphisms of the mtDNA D-loop region of 158 individuals of silver carp(Hypophthalmichthys molitrix) were analyzed with PCR technique.The wild samples were collected from Yangtze River drainage area(Jianli section in Hubei Province,Ruichang section in Jiangxi Province and Changsha section in Hunan Province) respectively.The artificially propagated population,the sixth generation,originated from Yangtze River 1957,were collected from hatchery station in Ninghe area,Tianjin City.The D-loop regions amplified were digested with 14 restriction endonucleases.The results showed that the length of the DNA fragments amplified in all samples were about 1.6 kb.The restriction sites were observed with 10 enzymes(AfaⅠ,AluⅠ,DraⅠ,EcoRⅠ,HaeⅢ,HapⅡ,HinfⅠ,MboⅠ,TaqⅠand XspⅠ) and 16 haplotypes were detected.HaplotypeⅠoccupied 57.5%~90.0% in four populations.The haplotype diversity(h) and nucleotide diversity(π) in four populations were 0.1885~0.6526 and 0.001182~0.007570.The Rogers′ genetic distance between Jianli and Ruichang populations was the shortest(0.1250),while the farthest distance was between Jianli and Ninghe(0.2693).The χ2 test showed that there was significant variance among four populations.The haplotype diversity(h) and nucleotide diversity(π) in Ninghe population were both the highest and pattern C of HinfⅠwas unique in Ninghe population,occupying 20%.

相似文章

低氧胁迫对鲢抗氧化酶活性及SODs基因表达的影响淡水渔业 2021,51 (3) 53-59
傀儡湖鲢、鳙的生长特性及渔业利用 [朱峰跃, 刘家寿, 李钟杰, 张堂林, 叶少文, 苑晶, 郭志强, 张超文] 淡水渔业 2013,43 (2) 32-35
鲢IL-8基因的克隆与表达分析 [孙军, 梁卉, 林小涛, 胡云凤, 童芳] 淡水渔业 2009,39 (2) 27-32
长江宜昌江段和东洞庭湖鲢、鳙饵料生物组成及季节性变化 [潘文杰, 郑永华, 高雷, 刘绍平, 段辛斌, 陈大庆] 淡水渔业 2017 (6) 32-39
滤食性鱼类鲢放养量对淀山湖水质影响的围隔试验 [张宁, 季高华, 王丽卿] 淡水渔业 2012,42 (5) 85-90
鲢早期发育对过饱和总溶解气体响应与仔鱼耐受性的研究 [杨传顺, 吴湘香, 杜开开, 柳凌, 茹辉军, 张燕, 倪朝辉, 李云峰] 淡水渔业 2019 (1) 50-56
鲢鳙在长寿湖水生态系统氮磷循环中的作用 [李晓洁, 唐敏, 李云, 叶勤, 薛洋, 靳涛, 祖学勤] 淡水渔业 2018 (3) 40-46
鲢肠道淀粉酶的分离纯化及部分性质研究 [郭彩华, 张希春, 卢珍华, 郑彩梅] 淡水渔业 2007,37 (3) 34-36
不同运动模式下鲢幼鱼游泳特性研究 [郝文超, 王从锋, 刘慧杰, 王瑶, 冯三杰] 淡水渔业 2018 (2) 3-9+15
1株鲢病原弧菌的分离鉴定 [孙翰昌] 淡水渔业 2009,39 (4) 45-48+44

导出引用

计量

文章访问数: 7

HTML全文浏览量: 0

PDF下载量: 0

4个群体鲢mtDNA D-loop的PCR-RFLP分析

相似文章

所属期刊

淡水渔业

推荐期刊

淡水渔业

黑龙江水产