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当前位置: 首页 > 文章 > 黑果腺肋花楸原花青素的纯化及表儿茶素和原花青素B_2含量测定 食品工业科技 2017,38 (19) 240-244
Position: Home > Articles > Purification of black chokeberry procyanidins and determination of epicatechin and procyanidins B_2 by HPLC Science and Technology of Food Industry 2017,38 (19) 240-244

黑果腺肋花楸原花青素的纯化及表儿茶素和原花青素B_2含量测定

作  者：
朱月;张海平+;侯亚男;安建辉;封晓茹;温馨亚;李腾;孙爱东
单  位：
北京林业大学生物科学与技术学院食品加工与安全北京市重点实验室
关键词：
黑果腺肋花楸;AB-8大孔树脂;原花青素;高效液相色谱(HPLC);表儿茶素;原花青素B_2
摘  要：
本文研究AB-8型大孔树脂对黑果腺肋花楸原花青素粗提物的吸附和解吸特性,并利用高效液相色谱法测定原花青素纯化物中表儿茶素和原花青素B_2含量。结果表明,上样溶液浓度4 mg/m L,吸附流速2 BV/h,解吸液乙醇浓度为50%,得到纯化物中原花青素含量为14.29%;高效液相色谱法采用Aglient Eclipse XDB-C18色谱柱(4.6 mm×250 mm,5μm),以甲醇(A)-水(B)为流动相梯度洗脱(0~10 min,10%A-30%A;10~20 min,30%A-70%A;20~30 min,70%A-100%A),流速1 m L/min,检测波长280 nm,表儿茶素和原花青素B2质量浓度在0.025~0.2 mg/m L内呈现良好的线性关系,两种成分的加样回收率分别为105.5%和99.8%;经测定黑果腺肋花楸纯化物中表儿茶素和原花青素B_2含量分别为14.26 mg/g和22.45 mg/g。
译  名：
Purification of black chokeberry procyanidins and determination of epicatechin and procyanidins B_2 by HPLC
作  者：
ZHU Yue;ZHANG Hai-ping+;HOU Ya-nan;AN Jian-hui;FENG Xiao-ru;WEN Xin-ya;LI Teng;SUN Ai-dong;Key Loboratory of Forestry Food Processing Security,College of Biological Sciences and Biotechnology,Beijing Forestry University;
单  位：
Key Loboratory of Forestry Food Processing Security,College of Biological Sciences and Biotechnology,Beijing Forestry University
关键词：
black chokeberry;;AB-8 macroporous resins;;procyanidins;;high performance liquid chromatography;;epicatechin;;procyanidins B2
摘  要：
The adsorption and desorption properties of procyanidins from black chokeberry with AB-8 macroporous resins was studied. And the HPLC method was used to determine the content of epicatechin and procyanidins B_2 in procyanidins purification.The results showed that,procyanidins concentration of the sample solution was 4.0 mg/m L,adsorption flow rate was 2 BV/h,the desorption concentration of ethanol was 50%. Under the proposed conditions,the purity of procyanidins content from black chokeberry was 14.29%. Chromatographic separation was performed on the Aglient Eclipse XDB-C18,4.6 mm ×250 mm. The mobile phase was methanol( A)-water( B) with the gradient elution( 0 ~10 min,10% A-30% A; 10 ~20 min,30% A-70% A; 20 ~ 30 min,70% A-100% A). The flow rate was 1.0 m L/min and the detective wavelength was set at280 nm. The linear ranges of epicatechin and procyanidins B2 concentration were 0.025~0.2 mg/m L.The sample recoveries rate of the two components were 105.5% and 99.8% respectively. The content of epicatechin and procyanidins B_2 in black chokeberry procyanidins purification were 14.26 mg/g and 22.45 mg/g,respectively.

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