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当前位置: 首页 > 文章 > 湘华鲮暴发性死亡病原菌的分离与鉴定 云南农业大学学报(自然科学) 2020 (3) 443-449
Position: Home > Articles > Isolation and Identification of Pathogens from an Outbreak Deaths of Sinilabeo decorus tungting Journal of Yunnan Agricultural University(Natural Science) 2020 (3) 443-449

湘华鲮暴发性死亡病原菌的分离与鉴定

作  者：
邓时铭;程小飞;刘丽;邹利;李传武;蒋国民
单  位：
关键词：
湘华鲮;嗜水气单胞菌;维氏气单胞菌;病原菌;分离与鉴定
摘  要：
【目的】查明池塘养殖湘华鲮暴发性死亡的原因,并对分离的病菌进行鉴定和回归感染试验。【方法】取濒死湘华鲮病变组织进行细菌分离纯化,采用细菌形态特征、生化特性及16S rDNA基因系统进化树分析,进行优势菌的鉴定,并通过人工感染和药敏试验评价优势菌的毒力和药物敏感性。【结果】分离获得优势菌XHL-01和XHL-02,经鉴定分别为嗜水气单胞菌和维氏气单胞菌;感染结果表明:两菌株对湘华鲮均具有致病性,感染发病症状与临床症状类似;药敏结果显示:两菌株对青霉素、氨苄西林、克林霉素和万古霉素产生耐药,对头孢噻肟、头孢他啶和多西环素等9种药物高度敏感。【结论】从发病湘华鲮中分离获得的致病菌XHL-01和XHL-02对湘华鲮都具有致病性,可能为此次暴发性死亡的细菌性病原,药物敏感结果可为该病的防控提供指导。
译  名：
Isolation and Identification of Pathogens from an Outbreak Deaths of Sinilabeo decorus tungting
作  者：
DENG Shiming;CHENG Xiaofei;LIU Li;ZOU Li;LI Chuanwu;JIANG Guomin;Fisheries Research Institute of Hunan Province;The Aquatic Seed Farm of Hunan Province;Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province;
关键词：
Sinilabeo decorus tungting(Nichols);;Aeromonas hydrophila;;Aeromonas veronii;;pathogen;;isolation and identification
摘  要：
[Purpose]To find out the cause of outbreak deaths of Sinilabeo decorus tungting in pond culture, the isolation, purification, identification and artificial infection test of pathogenic bacteria were carried out.[Method]The dominant bacteria were isolated and purified from the diseased tissues of the dying S. decorus tungting. The dominant bacteria were identified through morphological characteristics, physicochemical characteristics and 16 S rDNA sequence analysis. And the toxicity and drug sensitivity of dominant bacteria were evaluated by artificial infection and drug sensitivity test.[Result]The dominant bacteria XHL-01 and XHL-02 were isolated and identified as Aeromonas hydrophila and A. veronii, respectively. The results of artificial infection test showed that the symptoms of artificial infection S. decorus tungting were similar to clinical symptoms, so the two strains were pathogenic to S. decorus tungting. The drug sensitivity results showed that the two strains were resistant to penicillin, ampicillin, clindamycin and vancomycin, but highly sensitive to 9 drugs such as cefotaxime, ceftazidime, doxycycline.[Conclusion]The pathogenic bacteria XHL-01 and XHL-02 isolated from the diseased S. decorus tungting are pathogenic to S. decorus tungting, which may be the bacterial pathogen of the outbreak death. The drug sensitive results can provide guidance for the prevention and control of the disease.

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