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Position: Home > Articles > Study on the Contamination Control Effect of Virus-free Potato Plantlets Northern Horticulture 2011 (20) 134-136

马铃薯脱毒试管苗污染控制效果研究

作  者:
康萍芝;张丽荣;詹虹;沈瑞清;郭成瑾
单  位:
宁夏农林科学院植物保护研究所;宁夏马铃薯脱毒种薯繁育中心
关键词:
马铃薯脱毒苗;污染;抑菌剂;控制效果
摘  要:
通过在MS培养基中加入抗生素和杀菌剂进行逐步筛选,解决马铃薯脱毒试管苗快繁过程中的污染问题。结果表明:防止马铃薯脱毒试管苗污染的最佳抑菌剂组合MS+50mg/L硫酸庆大霉素+50mg/L氨苄青霉素+50mg/L硫酸链霉素+0.4g/L多菌灵,该组合有效降低了马铃薯脱毒苗细菌和真菌污染,提高了快繁效率。
译  名:
Study on the Contamination Control Effect of Virus-free Potato Plantlets
作  者:
KANG Ping-zhi1,ZHANG Li-rong1,ZHAN Hong2,SHEN Rui-qing1,GUO Cheng-jin1(1.Institute of Plant Protection,Ningxia Academy of Agriculture and Forestry Sciences,Ningxia Key Laboratory for Control of Plant Disease and Insect Pest,Yinchuan,Ningxia 750002;2.Ningxia Propagation Center of Virus-free Seed Potatoes,Pingjipu,Ningxia 750024)
关键词:
virus-free potato plantlets;contamination;antibacterial agent;control effect
摘  要:
In order to solve contamination problem of virus-free potato plantlets in rapid propagation,this experiment by joining antibiotics and fungicide in MS medium gradually screened and choosed.The results showed that best antibacterial agent preventing the virus-free potato plantlets contamination,namely,MS+50 mg/L Gentamycin Sulfate+50 mg/L Ampicillin Sodium+50 mg/L Streptomycin Sulfate+0.6 g/L carbendazim.Through this way,bacteria and fungi contamination of virus-free potato plantlets were reduced effectively and efficiency of rapid propagation could alse be improved.

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