作 者：
张美荣;廖想想;陈丹;毛永江;刘坤;陈亮;王杏龙;杨章平;杨利国
单 位：
扬州大学动物科学与技术学院;华中农业大学动物遗传育种与繁殖教育部重点实验室;上海牛奶集团海丰奶牛场有限公司
关键词：
澳系进口荷斯坦牛;CXCR1基因;泌乳性状;遗传多态性;PCR-SSCP;SCS
摘 要：
采用聚合酶链式反应-单链构象多态性(PCR-SSCP)技术对以海丰奶牛场588头澳系进口荷斯坦牛牛趋化因子受体1(Chemokine receptor 1,CXCR1)基因的遗传多态性进行分析;采用混合动物模型分析CXCR1基因2个突变位点与测定日产奶量、乳脂率、乳蛋白率、305d产奶量、305d乳脂量、305d乳蛋白量及体细胞评分7个性状的相关性,寻找可用于生产实际的分子标记。结果显示,CXCR1基因5′侧翼区-1830位点发生了A→G的突变,检测到AA、AG和GG 3种基因型,频率分别为0.684、0.289和0.027,等位基因A和G的频率分别为0.828和0.172;GG基因型奶牛的日产奶量、SCS和305d产奶量均极显著高于AA基因型(P<0.01),而其乳脂率和乳蛋白率却显著低于AA基因型奶牛(P<0.05)。编码区783位点仅发现AA和AC 2种基因型,基因型频率分别为0.886和0.114,等位基因A和C的频率分别为0.943和0.057。AA型的个体的日产奶量、305d脂肪产量和305d蛋白产量极显著高于AC型个体,而其乳脂率、乳蛋白率和SCS却极显著低于AC型个体(P<0.01)。结果表明,CXCR1基因遗传突变对澳系进口荷斯坦牛泌乳性状和乳房炎抗性有较大的遗传效应,可用于澳系进口荷斯坦牛的分子标记辅助选择。
译 名：
Genetic polymorphism of the CXCR1 gene and its associations with milk traits and SCS in Holstein imported from Australia
作 者：
ZHANG Mei-rong;LIAO Xiang-xiang;CHEN Dan;MAO Yong-jiang;LIU Kun;CHEN Li-ang;WANG Xing-long;YANG Zhang-ping;YANG Li-guo;Animal Science and Technology College,Yangzhou University;China Education Ministry's Key Laboratory in Agricultural Animal Genetics,Breeding and Reproduction,College of Animal Science and Technology,Huazhong Agricultural University;Haifeng Dairy Cattle Farm,Shanghai Dairy Group;
关键词：
Holstein imported from Australia;;CXCR1gene;;milk traits;;genetic polymorphism;;PCR-SSCP;;SCS
摘 要：
The effects of two mutations in Chemokine receptor 1(CXCR1)gene for 588Holstein cows imported from Australia in Haifeng dairy cattle farm were investigated according to 7milking performances:tested day milk yield,milk fat percentage,milk protein percentage,305dcorrected milk yield,305dmilk fat yield,305dmilk protein yield and somatic cell score(SCS).The aim was to explore the significant molecular marker in practical dairy production.The polymorphism of CXCR1gene was analyzed using polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP)technique,and with a mixed animal model,to verify the effects of the polymorphism on milking performances and SCS.The results showed three genotypes were identified in the 5′upstream of-1830(A/G),and the genotypic frequencies of AA,AG and GG were0.684,0.289and 0.027,respectively.The gene frequencies of A and G were 0.828and 0.172.GG genotype had significantly higher tested day milk yield,SCS and 305dcorrected milk yield than AA genotype(P<0.01),but its milk fat percentage,milk protein percentage were lower than AA(P<0.05).Only two genotypes were found in the coding region at 783site,and the genotypic frequencies of AA and AC were 0.886and 0.114,respectively.The gene frequencies of A and C were 0.943and 0.057.The tested day milk yield,305dmilk fat yield and 305dmilk protein yield of AA genotype were significantly higher than AC genotype.However,AA genotype had significantly lower milk fat percentage,milk protein percentage and SCS than AC genotype(P<0.01).The CXCR1gene genetic diversity has a great genetic effect on milk traits and mastitis resistance and could be a useful genetic marker for Holstein cows imported from Australia.
