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Position: Home > Articles > Pyrogenic Effect of LPS Infusion into Veins and Liver Protein Analysis by SDS-PAGE in Rabbits Hubei Agricultural Sciences 2011,50 (7) 130-133

静脉灌注LPS对家兔的发热效应及肝蛋白的SDS-PAGE比较分析

作  者:
张世栋;李世宏;王东升;龚成珍;李宏胜;李锦宇;荔霞;严作廷
单  位:
甘肃农业大学动物医学院;中国农业科学院兰州畜牧与兽药研究所/中国农业科学院临床兽医学研究中心/甘肃省中兽医工程技术研究中心
关键词:
LPS;发热模型;白虎汤;SDS-PAGE;蛋白差异表达
摘  要:
以静脉注射内毒素(LPS)的方法建立了家兔发热模型,并以白虎汤的降温治疗作为反证,再运用SDS-聚丙烯酰胺凝胶电泳比较和分析了LPS引起的发热效应对家兔肝脏总蛋白表达变化的影响以及肝组织病理变化。结果表明,以15μg/kg体重的LPS静脉注射家兔能很好地复制出动物温病模型,模型组、治疗组和对照组3组动物的肝蛋白表达均有各自的特异条带。所以,肝组织蛋白的表达变化可以作为动物温病研究与评价的生物学指标。
译  名:
Pyrogenic Effect of LPS Infusion into Veins and Liver Protein Analysis by SDS-PAGE in Rabbits
作  者:
ZHANG Shi-dong1,LI Shi-hong1,WANG Dong-sheng1,GONG Cheng-zhen2,LI Hong-sheng1, LI Jin-yu1,LI Xia1,YAN Zuo-ting1(1.Lanzhou Institute of Animal & Veterinary Pharmaceutics,The Chinese Academy of Agricultural Sciences / Research Center of Clinical Veterinary Medicine,CAAS/Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province,Lanzhou 730050,China;2.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China)
关键词:
LPS;fever model;Baihu Soup;SDS-PAGE;diversity of protein expression
摘  要:
The pyrogenic effect induced by LPS infusion into veins was analysized and Baihu Soup was used to reduce fever as counterevidence.The changing of the liver protein expression and pathological changes of liver tissue caused by LPS fever were compared by SDS-PAGE.The results indicated that the fever model can be replicated by 15μg/kg LPS infusion into veins in rabbits,and there were characteristic molecular-weight proteins in control,LPS-infusion and Baihu Soup treated animals,respectively.The diversity of liver protein expression,therefore,could be a biological parameter of animal fever study and estimation.

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