作 者：
殷彬;林仪彤;迟淑艳;谭北平;姚红梅;姚亚军;董晓慧;杨奇慧;刘泓宇;章双
单 位：
广东海洋大学水产动物营养与饲料实验室;南海生物资源开发与利用协同创新中心;长沙兴嘉生物工程股份有限公司
关键词：
珍珠龙胆;锌源;生长性能;抗氧化能力;肠道;矿物元素沉积;相对生物学效价
摘 要：
为研究硫酸锌(ZnSO_4·7H_2O)、碱式硫酸锌[ZnSO_4·3Zn(OH)2·5H_2O]、甘氨酸锌(C_4H_8N_2O_4Zn)和羟基蛋氨酸锌(C_5H_(11)NO_6S_2Zn)对珍珠龙胆生长性能、肝脏抗氧化能力、肠道发育以及脊椎骨矿物元素沉积的影响,实验选取体质量为(11.00±0.12)g珍珠龙胆360尾,随机分为4组(每组3个重复,每个重复30尾),分别添加ZnSO_4·7H_2O、ZnSO_4·3Zn(OH)2·5H_2O、C4H8N2O4Zn和C5H11NO6S2Zn,记作ZnSO_4、Zn_4(OH)_6SO_4、Zn-Gly和Zn-MHA,使饲料锌水平分别为118.85、116.92、120.66、119.23 mg/kg,制成等氮等脂的实验饲料,饲养8周。结果显示,Zn_4(OH)_6SO_4、Zn-Gly和Zn-MHA组增重率和特定生长率均显著高于ZnSO_4组。Zn_4(OH)_6SO_4、Zn-Gly、Zn-MHA组饲料系数均显著低于ZnSO_4组。Zn-Gly、ZnMHA组肝脏丙二醛含量显著低于ZnSO_4和Zn_4(OH)_6SO_4组,Zn-MHA组Cu Zn-SOD和TSOD活性均显著高于其余各组。与ZnSO_4相比,Zn_4(OH)_6SO_4、Zn-Gly和Zn-MHA均显著提高了前肠和中肠的皱襞厚度;Zn-Gly和Zn-MHA组后肠肌层显著厚于ZnSO_4和Zn_4(OH)_6SO_4组。Zn-Gly组珍珠龙胆脊椎骨Fe和Mn含量显著高于ZnSO_4组。相对于ZnSO_4,以增重率和肝脏CuZn-SOD含量为评价指标时,Zn_4(OH)_6SO_4、Zn-Gly和ZnMHA的相对生物学效价(RBV)分别为119.15%、110.90%,118.43%、113.58%和122.45%、130.84%。研究表明,与ZnSO_4相比,Zn_4(OH)_6SO_4能够显著提高珍珠龙胆幼鱼的生长性能,提高肝脏SOD活性,增强脊椎骨中Fe的沉积;Zn-Gly和Zn-MHA能够显著提高珍珠龙胆幼鱼的生长性能,促进肠道发育,调节脊椎骨矿物元素沉积。
译 名：
Effects of four forms of zinc on growth performance, antioxidant capacity and vertebrae mineral accumulation of juvenile hybrid grouper(Epinephelus fuscoguttatus♀ × E. lanceolatu)♂
作 者：
YIN Bin;LIN Yitong;CHI Shuyan;TAN Beiping;YAO Hongmei;YAO Yajun;DONG Xiaohui;YANG Qihui;LIU Hongyu;ZHANG Shuang;Laboratory of Aquatic Animal Nutrition and Feed, Guangdong Ocean University;South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center;Changsha Xingjia Biotechnology Engineering Co.Ltd.;
关键词：
Epinephelus fuscoguttatus♀×E. lanceolatu♂;;zinc sources;;growth performance;;antioxidant capacity;;intestine;;mineral accumulation;;relative bioavailability values
摘 要：
This study was to investigate the effects of four forms of zinc on growth performance, antioxidant capacity, intestinal performance and vertebrae mineral accumulation of juvenile hybrid grouper(Epinephelus fuscoguttatus♀×E. lanceolatu♂). The experimental diets were isonitrogenous and isolipidic diets by adding zinc sulfate(ZnSO_4), basic zinc sulfate [Zn_4(OH)_6SO_4], zinc glycine(Zn-Gly) and zinc hydroxymethionine(Zn-MHA), respectively. The zinc content of the four diets was 118.85, 116.92, 120.66 and 119.23 mg/kg, respectively. Three hundred and sixty individuals of grouper [initial average weight(11±0.12) g] were randomly divided into four groups with three replicates at a stock density of thirty, and fed with test diets for 8 weeks. The results showed that the weight gain rate and specific growth rate in Zn_4(OH)_6SO_4, Zn-Gly and Zn-MHA groups were significantly higher than those in ZnSO_4 group. The feed coefficient rate of fish fed Zn_4(OH)_6SO_4, Zn-Gly and Zn-MHA groups were significantly lower than that fed ZnSO_4 group. The survival rate was no different among the four groups.Hepatic malonaldehyde contents in Zn-Gly and Zn-MHA groups were significantly decreased compared with ZnSO_4 and Zn_4(OH)_6SO_4 groups. The activity of copper-zinc superoxide dismutase in the liver of fish fed with ZnMHA was significantly higher than that in other groups. Compared to ZnSO_4 group, plica heights of foregut and midgut of fish fed with Zn_4(OH)_6SO_4, Zn-Gly and Zn-MHA were significantly increased. The muscle thickness of hindgut in the Zn-Gly and Zn-MHA groups was significantly higher than that in ZnSO_4 and Zn_4(OH)_6SO_4 groups.Vertebrae iron and manganese contents in fish fed with Zn-Gly were significantly higher than those in ZnSO_4 group. According to weight gain rate and hepatic copper-zinc superoxide dismutase contents, the relative bioavailability values of Zn_4(OH)_6SO_4, Zn-Gly and Zn-MHA, compared to ZnSO_4, were 119.15% and 110.90%, 118.43%and 113.58%, 122.45% and 130.84%, respectively. In conclusion, in the aspects of growth performance, activity of hepatic SOD and the iron accumulation in the vertebrae, Zn_4(OH)_6SO_4 is better than ZnSO_4. However, Zn-Gly or Zn-MHA as organic form of zinc can impove growth performance, oxidation resistance, and intestine development compared to ZnSO_4.
