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Position: Home > Articles > Isolation and determination of silver-resistant bacteria plasmids. Chinese Journal of Applied Ecology 2006,17 (2) 305-308

银离子抗性细菌质粒的分离与鉴定

作  者:
李钧敏;金则新
单  位:
台州学院生态研究所
关键词:
细菌;质粒;银离子;抗性
摘  要:
通过对不同化工厂废水处理池的活性污泥进行富集、不同浓度银离子驯化后,分离得到30株银离子抗性细菌,最大硝酸银耐受量可达80mg.ml-1.对这些细菌进行质粒抽提与鉴定,质粒检出率为76.67%.40mmol.L-1的苯甲酸钠对HAg4细菌质粒的消除率可达98.75%;而350μg.ml-1的吖啶橙对HAg4细菌质粒的消除率只有77.78%.细菌质粒与银离子抗性密切相关.
译  名:
Isolation and determination of silver-resistant bacteria plasmids.
作  者:
LI Junmin,JIN Zexin (Ecology Institute of Taizhou University,Linhai 317000,China)
关键词:
Bacteria, Plasmid, Silver, Resistance.
摘  要:
Through the enrichment of the active mud obtained from three chemical plants and the domestication with different concentration Ag+ solution,thirty bacteria strains with silver (Ag+)-resistance were isolated,among which,the highest Ag+-resistant concentration was 80 mg·ml -1 .The plasmids in these bacteria were extracted,with the detection rate of 76.67%.The elimination rate of the plasmid in HAg4 bacteria was 98.75% by 40 mmol·L -1 sodium benzoate,and 77.78% by 350 μg·ml -1 acridine orange.It was suggested that the Ag+-resistance of bacteria was highly correlated with their plasmids.

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