单 位：
吉林农业大学动物科学技术学院;长春理工大学生命科学技术学院
关键词：
嗜酸乳酸杆菌;自主活动受限;应激;肠道黏膜免疫
摘 要：
目的:探讨嗜酸乳酸杆菌(Lactobacillus acidophilus,LA)对自主活动受限型应激小鼠肠道黏膜免疫状态的影响。方法:以健康雌性BALB/c小鼠为受试动物,分为常规饲养组(NC组)、常规饲养条件灌胃LA组(NC+LA组)、应激组(S组)、应激条件灌胃LA组(S+LA组),小鼠的LA灌胃剂量为10~8CFU/d;应激组小鼠每天被限制自主活动3 h;15 d后取材,采用流式细胞术、酶联免疫吸附(enzyme-linked immunosorbem assay,ELISA)等方法,对肠系膜淋巴结(mesenteric lvmph nodes,MLN)中CD4~+T细胞、CD8~+T细胞和CDllc~+树突细胞(CDllc~+dendritic cell,CDllc~+DC)比例,结肠组织白细胞介素-10(intefleukin-10,IL-10)和白细胞介素-17(intefleukin-17,IL-17)水平,以及小肠总分泌型免疫球蛋白A(secreted immunoglobulin A,sIgA)水平进行检测。结果:常规饲养条件下,LA能极显著提高小鼠MLN中CD4~+T细胞比例(P<0.01),显著提高MLN中CDllc~+DC比例、小肠总sIgA水平和结肠组织IL-10水平(P<0.05),显著降低结肠组织IL-17水平(P<0.05),而对MLN中CD8~+T细胞比例无显著影响(P>0.05);应激条件能极显著降低小鼠MLN中CD4~+T细胞比例和CDllc~+DC比例(P<0.01),显著降低CD8~+T细胞比例(P<0.05),极显著降低小鼠结肠组织IL-10水平和小肠总sIgA水平(P<0.01),极显著提高结肠组织IL-17水平(P<0.01);应激条件下,LA能显著提高小鼠MLN中CD4~+T细胞比例和CDllc~+DC比例(P<0.05),显著提高小鼠结肠组织IL-10水平和小肠总sIRA水平(p<0.05),并显著降低结肠组织IL-17水平(p<0.05),而对小鼠MLN中CD8~+T细胞比例无显著影响(P>0.05)。结论:给予自主活动受限型应激小鼠LA干预,可提高小鼠MLN中CD4~+T细胞和CDllc~+DC比例,上调结肠IL-10和小肠总sIgA分泌水平,下调结肠IL-17分泌水平,调节应激小鼠的肠道黏膜免疫状态。
译 名：
Influence of Lactobacillus acidophilus on Intestinal Mucosal Immunity in a Mouse Model of Stress Induced by Auto-Activity Restriction
作 者：
HAO Fengqi;LI Jingmei;YANG Guilian;School of Life Science and Technology,Changchun University of Science and Technology;College of Animal Science and Technology,Jilin Agricultural University;
关键词：
Lactobacillus acidophilus;;auto-activity restriction;;stress;;intestinal mucosal immunity
摘 要：
Objective:To assess the influence of Lactobacillus acidophilus on intestinal mucosal immunity in a mouse model of stress induced by auto-activity restriction.Methods:Male health BALB/c mice were divided into 4 groups.In normal control group(NC),the mice received balanced diet and water and were left undisturbed in their cages.In NC plus Lactobacillus acidophilus(NC + LA) group,the mice were given a suspension of LA with 10~8 CFU/d per mouse through intragastric administration under normal control condition.In stress group(S),the mice were subjected to stress induced by auto-activity restriction for 3 h per day.In stress plus LA(S+LA) group,the mice were given a suspension of LA with 10~8 CFU/d per mouse through intragastric administration under stress condition.After 15 days,mice from each group were sacrificed.CD4~+T,CD8~+T and CD11c~+ dendritic cells(CD11c~+ DCs) in mesenteric lymph nodes(MLN),interleukin-10(IL-10) and interleukin-17(IL-17) levels in colon tissue,total secretory immunoglobulin A(slgA) level in small intestine were analyzed by flow cytometry and enzyme-linked immunosorbent assay(ELISA).Results:Under NC condition,the proportions of CD4~+T cells and CD11c~+DCs in MLN(CD4~+T cell:P < 0.01,CD11c~+DC:P < 0.05),small intestinal sIgA level and IL-10 level in colon tissue(sIgA,IL-10:P < 0.05) were enhanced by LA and IL-17 level in colon tissue was decreased(P < 0.05),but the proportion of CD8~+T cells in MLN was not significantly varied(P > 0.05).After auto-activity restriction,the proportions of CD4~+T cells,CD8~+T cells and CD11c~+DCs,and the levels of IL-10 and small intestinal sIgA were decreased(CD4~+T cell,CD11c~+DC,IL-10,sIgA:P < 0.01,CD8~+T cell:P < 0.05).Interestingly,the proportion of CD4~+T cells and CD11c~+DCs,the levels of IL-10 and small intestinal sIgA were increased,and the level of IL-17 was decreased by LA under stress condition(P < 0.05),but the proportion of CD8~+T cells was not significantly affected(P > 0.05).Conclusion:LA administered to mice subjected to auto-activity restriction enhances the proportions of CD4~+T cells and CD11c~+DCs in MLN,up-regulates the levels of IL-10 in colon and total small intestinal sIgA,and downregulates the level of IL-17 in colon,thereby modulating their intestinal mucosal immunity under stress condition.
