中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 高温胁迫对Bt棉铃壳中Bt蛋白含量及氮代谢的影响 应用生态学报 2015,26 (10) 3202-3206
Position: Home > Articles > Effects of high temperature on Bt protein content and nitrogen metabolic physiology in boll wall of Bt cotton Chinese Journal of Applied Ecology 2015,26 (10) 3202-3206

高温胁迫对Bt棉铃壳中Bt蛋白含量及氮代谢的影响

作  者：
王俊;Eltayib H.M.A.Abidallah;花明明;衡丽;吕春花;陈德华
单  位：
扬州大学江苏省作物遗传生理重点实验室
关键词：
Bt棉;高温;Bt蛋白;氮代谢
摘  要：
以Bt基因来源于中国的棉花品种泗抗1号(常规种)、泗抗3号(杂交种)和来源于美国的棉花品种99B(常规棉)、岱杂1号(杂交棉)为材料,研究了不同高温水平下Bt棉盛铃期铃壳中Bt蛋白含量变化及氮代谢生理特征.结果表明:铃壳中Bt蛋白含量随温度升高而降低,与对照相比(32℃),常规棉品种在38℃、杂交棉品种在40℃以上时,铃壳中Bt蛋白含量大幅度下降.其中,常规种泗抗1号和99B在38℃时分别下降53.0%和69.5%;杂交种泗抗3号和岱杂1号在40℃时下降64.8%和54.1%.铃壳Bt杀虫蛋白含量下降显著时,其可溶性蛋白含量明显下降,游离氨基酸含量明显提高,GPT活性显著下降,蛋白酶活性显著增加.高温影响铃壳的氮代谢引起Bt蛋白的分解加剧,合成减弱,从而造成Bt蛋白含量减少,抗虫性下降.
译  名：
Effects of high temperature on Bt protein content and nitrogen metabolic physiology in boll wall of Bt cotton
作  者：
WANG Jun;Eltayib H.M.A.Abidallah;HUA Ming-ming;HENG Li;LYU Chun-hua;CHEN De-hua;Jiangsu Province Key Laboratory of Genetics and Physiology,Yangzhou University;Faculty of Forestry,Department of Forest Management,Khartoum University;
关键词：
Bt cotton;;high temperature;;Bt protein;;nitrogen metabolic physiology
摘  要：
Bt cotton cultivar Sikang 1( a conventional cultivar) and Sikang 3( a hybrid cultivar)from China,and 99B( a conventional cultivar) and Daiza 1( a hybrid cultivar) from USA were selected as experimental materials,the ball wall Bt protein content and nitrogen metabolic physiology were investigated under different high temperature levels at peak boll stage. The results showed that the Bt protein content of boll wall decreased with the increasing temperature. Compared with the control( 32 ℃),the boll wall Bt protein content decreased significantly when the temperature was above 38 ℃ for the conventional cultivars and above 40 ℃ for the hybrid cultivars. The Bt protein contents of cultivar Sikang 1 and 99 B decreased by 53. 0% and 69. 5% respectively with the temperature at 38 ℃,and that of cultivar Sikang 3 and Daiza 1 decreased by 64. 8% and 54. 1% respectively with the temperature at 40 ℃. Greater reductions in the boll wall soluble protein contents and GPT activities,larger increments for the boll wall free amino acid contents and proteinsase activities were also observed when the boll wall Bt protein content was significantly reduced. Therefore,high temperature resulted in the reduction of Bt protein synthesis and increase of the insecticidal protein degradation in the boll wall significantly,which caused the reductions in boll wall Bt protein content and insect resistance.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46