当前位置: 首页 > 文章 > 巨尾阿丽蝇幼期血淋巴可溶性蛋白组成的时间特征 浙江大学学报(农业与生命科学版) 2004,30 (1)
Position: Home > Articles > Development profile of hemolymph soluble proteins during the larval and pupal stages in Aldrichina grahami Journal of Zhejiang University (Agriculture and Life Sciences) 2004,30 (1)

巨尾阿丽蝇幼期血淋巴可溶性蛋白组成的时间特征

作  者:
朱光辉;叶恭银;胡萃
单  位:
浙江大学应用昆虫学研究所
关键词:
巨尾阿丽蝇;血淋巴;蛋白质电泳;生长发育
摘  要:
应用梯度SDS-聚丙烯酰胺凝胶(5%~20%)电泳,分析了24℃下巨尾阿丽蝇Aldrichinagra-hami幼虫和蛹期血淋巴可溶性蛋白组成的时间动态.结果表明:血淋巴中共出现12条清晰可见的蛋白带,其分子量范围为11~230kD.各蛋白带的含量在生长发育过程中呈一定规律变化,其中a带(230kD)和i带(20kD)的含量在幼虫期呈逐渐增加趋势,在蛹期呈逐渐降低趋势;产卵后3、4日龄幼虫的血淋巴具有独特的蛋白质电泳图谱.
译  名:
Development profile of hemolymph soluble proteins during the larval and pupal stages in Aldrichina grahami
作  者:
ZHU Guang-hui, YE Gong-yin, HU Cui(Institute of Applied Entomology, Zhejiang University, Hangzhou 310029, China)
关键词:
Aldrichina grahami; hemolymph; protein electrophoresis; development
摘  要:
The development profile of soluble hemolymph proteins was analyzed by the gradient SDS-polyacrylamide gel (5%-20%) electrophoresis during the larval and pupal stages of Aldrichina grahami (Aldrich) which was kept at 24 ℃. Twelve distinct component bands with a molecular mass range of approx. 11-230 kD were resolved. The quantity of each protein band changed with development in the larval and pupal stages acted a certain pattern. The quantities of band a (230 kD) and band i (20 kD) gradually increased during the larval stage and decreased gradually during the pupal stage. Hemolymph proteins from larvae three or four days after oviposition showed distinct age-specific electrophoretic profiles.

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