关键词：
橡胶树死皮;胶乳C-乳清;蛋白鉴定;双向凝胶电泳;MALDI-TOFMS
摘 要：
橡胶树死皮是影响天然橡胶产量的重要限制因子。通过双向凝胶电泳技术(2-DE)研究了橡胶死皮树与健康树胶乳C-乳清蛋白表达谱的差异。以软件分析后共获得31个差异表达的蛋白点,经基质辅助激光解析电离飞行时间质谱(MALDI-TOF MS)分析后,搜索NCBInr数据库,结果有10个蛋白点被成功鉴定,其中6个为未知蛋白,其余4个蛋白分别为chain A,struc-ture of ubiquitin-like protein,Rub1;β-1,3-glucanase;AIR9 protein和flagellar inner dynein arm heav-y chain 11。这些蛋白可能在橡胶树死皮发生过程中扮演重要角色。
译 名：
Identification and analysis of proteins in the latex C-serum related to the tapping panel dryness in Hevea brasiliensis
作 者：
ZHOU Xuemei1,2,YANG Lifu1,WANG Zhenhui1,ZOU Zhi1,YUAN Kun1(1.Rubber Research Institute,Key Laboratory of Biology and Genetic Resources of Rubber Tree,Ministry of Agriculture,Danzhou 571737,China;2.College of Agriculture,Hainan University,Haikou 570100,China)
关键词：
tapping panel dryness(TPD);latex C-serum;identification of protein;two-dimensional gel electrophoresis(2-DE protein);mass spectrometry(MALDI-TOF MS)
摘 要：
The tapping panel dryness(TPD) in Hevea brasiliensis is a significant limit factor influencing natural rubber production.In this experiment,the two-dimensional gel electrophoresis(2-DE) was used to study the differentiation of protein expression profiles of latex C-serum between TPD trees and healthy ones of H.brasiliensis.Thirty-one differentially expressed protein spots were obtained by software analysis.After these spots were subjected to matrix assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF MS) followed by searching for NCBInr database,ten spots were successfully identified.Six of them were identified as the unknown proteins and the remaining 4 were the chain A,structure of ubiquitin-like protein,Rub1;β-1,3-glucanase;AIR9 protein and flagellar inner dynein arm heavy chain 11.These proteins might play important roles in the process of TPD occuring.
