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当前位置: 首页 > 文章 > ELISA与HPLC-MS检测玉米中黄曲霉毒素B1的比较分析 中国饲料 2019 (9)
Position: Home > Articles > ELISA与HPLC-MS检测玉米中黄曲霉毒素B1的比较分析 China Feed 2019 (9)

ELISA与HPLC-MS检测玉米中黄曲霉毒素B1的比较分析

作  者：
陈琛;王炳彦;钟兴文;杨旭艳;马丹;戚敏;孙照程;陈莹;孔凡虎;华雪妃;章雨竹;陶琳丽;张曦
单  位：
云南省动物营养与饲料重点实验室
关键词：
玉米;黄曲霉毒素B1;检测;酶联免疫法;高效液相色谱-质谱法
摘  要：
本实验选用30份玉米样品,通过酶联免疫吸附(ELISA)法和高效液相色谱-质谱联用(HPLC-MS)法对玉米中黄曲霉毒素B1含量(AFB1)进行测定比较,得出两种方法的差异性和相关性.其中,使用HPLC-MS外标法定量时,设计两组标准曲线,分别是玉米基质液加标组曲线和纯标组曲线,得到的结果再与ELISA比较.结果显示:用ELISA筛选出的阳性样品,用HPLC-MS法确证亦为阳性,判定结果一致,但具体检出量存在差异.当样品中AFB1含量为2~6μg/kg时,测定结果 HPLC-MS(纯标组)
作  者：
CHEN Chen;WANG Bingyan;ZHONG Xingwen;YANG Xuyan;MA Dan;QI Min;SUN Zhaocheng;CHEN Ying;KONG Fanhu;HUA Xuefei;ZHANG Yuzhu;TAO Linli;ZHANG Xi;Faculty of Animal Science and Technology,Yunnan Key Laboratory of Animal Nutrition and Feed Science,Yunnan Agricultural University;Animal Husbandry and Veterinary Station of Xinqiao Town in Mouding County Chuxiong Prefecture of Yunnan Province;Animal Health Supervision Institute of Dali Bai Autonomous Prefecture;Veterinary Drug and Feed Monitoring Station of Yunnan Province;
单  位：
CHEN Chen%WANG Bingyan%ZHONG Xingwen%YANG Xuyan%MA Dan%QI Min%SUN Zhaocheng%CHEN Ying%KONG Fanhu%HUA Xuefei%ZHANG Yuzhu%TAO Linli%ZHANG Xi%Faculty of Animal Science and Technology,Yunnan Key Laboratory of Animal Nutrition and Feed Science,Yunnan Agricultural University%Animal Husbandry and Veterinary Station of Xinqiao Town in Mouding County Chuxiong Prefecture of Yunnan Province%Animal Health Supervision Institute of Dali Bai Autonomous Prefecture%Veterinary Drug and Feed Monitoring Station of Yunnan Province
关键词：
maize;;AFB1;;detect;;ELISA;;HPLC-MS
摘  要：
Thirty maize samples were selected in this experiment. AFB1 in maize was detected and compared by ELISA and HPLC-MS to get the differences and correlations between the two methods. The results showed that positive sample screened by ELISA were comfirmed by HPLC-MS,but there were differences in their detectable amount. The result was HPLC-MS(pure mark group) < ELISA < HPLC-MS(maize substrate liquid add mark group) when content aflatoxin B1 in sample was from 2 to 6 μg/kg,and the result of HPLC-MS(maize substrate liquid add mark group)≈ELISA or HPLC-MS(pure mark group) multiplied by 2 to 13. The result was ELISA < HPLC-MS(pure mark group)≈ HPLC-MS(maize substrate liquid add mark group) when content aflatoxin B1 in sample was from 11 to 50 μg/kg. And the result was HPLC-MS(pure mark group)≈HPLC-MS(maize substrate liquid add mark group)+ 3.6 μg/kg whose content aflatoxin B1 in sample lower than61.78 μg/kg. The results suggested that ELISA was a simple and rapid method with high accuracy,and it was suitable for fast screening of aflatoxin B1 in large number of maize samples. While HPLC-MS method takes longer and costs higher to detect,so it was more suitable for the confirmation of positive sample preliminarily screened by ELISA,and the accurate quantitative of aflatoxin B1 content.

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