Study on Inducting Barley(Hordeum vulgare) Haploids Using Hordeum Bulbosum Chromosome Elimination in H.Vulgare × H.Bulbosum Crosses

LI Shu-jie1,LI Jing-wen1,ZHANG Zheng-ying2(1.Institute of Biotechnology,Gansu Academy of Agricultural Science,Lanzhou,Gansu 730070,China; 2.Scientific Research Management Office,Gansu Academy of Agricultural Science,Lanzhou,Gansu 730070,China)

Chromosome elimination;Barley(Hordeum vulgare);Haploid inducing rate;Seed setting rate

Seven varieties of H.vulgare as maternal parents were crossed with a diploid H.bulbosum and a tetraploid H.bulbosum to induce haploids in their progeny,diploid H.bulbosum as male parents has on average 28.13% of the pollinated florets formed embryos,and the tetraploid H.bulbosum is 0.In the article,the effects of pollination time,daily maximum temperature during the pollination,and spraying exogenous hormones after pollination on seed setting rate is analysed.The result shows the highest seed setting rate will be gained by pollinating when two days after manual emasculation,spraying the mixed hormones of Dicamb∶2,4-D∶GA3=1∶2∶75 once a day during the first three days after pollination,and controlling the maximum temperature in the day during the pollination period less than 22 ℃.The reason of difference of seed setting rate between the year 2010 and 2011,effects of H.bulbosum genetype on haploid inducing rate are discussed.The objective of this article is to provide basic knowledge and practice guidelines in barely(Hordeum vulgare) haploids breeding.