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Position: Home > Articles > Isolation and Identification of Starch Granule-Associated Proteins(SGAPs) from Indica Rice FOOD SCIENCE 2018 (20) 240-245

籼米中淀粉粒结合蛋白的分离鉴定及提取工艺优化

作  者：
陈兰煊;杨阳;易翠平
单  位：
长沙理工大学化学与生物工程学院
关键词：
籼米;淀粉粒结合蛋白(SGAPs);高效液相色谱-串联质谱法;淀粉合成酶I(GBSS I);响应面优化
摘  要：
采用十二烷基硫酸钠(sodium dodecyl sulfate,SDS)缓冲体系分离提取碱洗籼米粉中的淀粉粒结合蛋白(starch granule-associated proteins,SGAPs),经高效液相色谱-串联质谱法鉴定表明:碱洗得到上、中、下层籼米淀粉中的SGAPs均主要为淀粉合成酶I(granule-bound starch synthase I,GBSS I),其他组分略有差别;其中以中层SGAPs含量最高,GBSS I达99.8%,谷蛋白质量分数仅为0.11%。采用响应面优化中层淀粉SGAPs的提取参数,得到最优条件为SDS添加量10.90%、煮沸时间4.10 min、MgSO4浓度0.90 mmol/L,此时SGAPs提取量为(1 028.61±0.05)μg/g。
译  名：
Isolation and Identification of Starch Granule-Associated Proteins(SGAPs) from Indica Rice
作  者：
CHEN Lanxuan;YANG Yang;YI Cuiping;School of Chemistry and Biological Engineering, Changsha University of Science and Technology;
单  位：
CHEN Lanxuan%YANG Yang%YI Cuiping%School of Chemistry and Biological Engineering, Changsha University of Science and Technology
关键词：
rice;;starch granule-associated proteins;;HPLC-MS/MS;;granule-bound starch synthase I;;response surface methodology
摘  要：
Starch granule-associated proteins(SGAPs) from indica rice were isolated by sodium dodecyl sulfate(SDS) buffer from alkali washed rice flour and then identified by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS). Results indicated that the SGAPs from the top, middle and bottom starch layers mainly consisted of granule-bound starch synthase I(GBSS I), which slightly varied with respect to other components. Among these layers, the content of SGAPs in the middle layer was the highest, with GBSS I accounting for up to 99.8% and gluten accounting for only 0.11% of the total. Using the response surface methodology, the optimized conditions for SGAPs extraction from the middle layer starch were obtained as follows: SDS 10.90%, boiling time 4.10 min and MgSO4 0.90 mmol/L. Under these conditions, the yield of SGAPs was(1 028.61 ± 0.05) μg/g.

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