译 名：
Study on Stem Apex Detoxification of Siraitia grosvenorii
作 者：
WU Qun-ying 1,LI Bo-lin 2,LI Jing-yun 3(1.Guilin Medical University,Guilin,Guangxi 541004;2.Guangxi Normal University,Guilin,Guangxi 541004;3.Guangxi Traditional Chinese Medical University,Nanning,Guangxi 530001)
关键词：
Luohanguo mosaic virus(WMV-2-Luo);stem apex detoxification;ELISA
摘 要：
Siraitia grosvenorii in vitro was used as material in this experiment to eliminate Luohanguo mosaic virus(WMV-2-Luo) by combing tissue culture of shoot-tip with heat therapy,and detected by indirect ELISA method and electron microscope.The results showed that the suitable time for heat treatment was 7～15days,the survival rate of tissue culture seedling was up to 90%～100%;0.2～0.5mm shoot-tip with heat treatment by 38.5℃for 10days of Siraitia grosvenorii were 100%free from WMV-2-Luo.
