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Position: Home > Articles > Pathogen Identification of Processing Chili Pepper Root Rot Disease in Xinjiang Northern Horticulture 2016 (15) 108-112

新疆加工辣椒根腐病病原鉴定

作  者:
方晓翠;屈荷丽;赵思峰;郭开发;马明
单  位:
石河子大学农学院绿洲农作物病害治理与植保资源利用自治区高校重点实验室
关键词:
新疆;加工辣椒;根腐病;尖孢镰刀菌;茄病镰刀菌
摘  要:
以制干辣椒根腐病病样为分离对象,采用形态学和分子生物学方法对致病菌进行鉴定。结果表明:共采集根腐病样211个,从中分离获得161个分离物,其中镰刀菌Fusarium spp.121个,占总分离物的75.16%;其它菌40个,占24.84%,说明镰刀菌是新疆加工辣椒的主要致病菌。经致病性测定后,采用形态学观察结合18SrDNA-ITS、EF-1α和β?tubulin 3个序列分析,将80株有致病性的镰刀菌鉴定为尖孢镰刀菌(F.oxysporum)和茄病镰刀菌(F.solani),其中尖孢镰刀菌57株,占镰刀菌分离物的47.11%;茄病镰刀菌23株,占19.01%。
译  名:
Pathogen Identification of Processing Chili Pepper Root Rot Disease in Xinjiang
作  者:
FANG Xiaocui;QU Heli;ZHAO Sifeng;GUO Kaifa;MA Ming;College of Agriculture,Shihezi University,Key Laboratory at the Universities of Xinjiang Uygur Autonomous Region for Oasis Agricultural Pest Management and Plant Protection Resource Utilization;
关键词:
Xinjiang;;processing chili pepper;;root rot disease;;Fusarium oxysporum;;Fusarium solani
摘  要:
To isolate and identify pathogen of processing chili pepper root rot disease in Xinjiang.A total of 211 samples were collected from different main production areas in Xinjiang.The pathogens which caused root rot of processing chili pepper were isolated and Fusariumisolates were identified according to the morphological features of culture and 18 S rDNA-ITS,EF-1α,β-tubulin gene sequences analysised of representative strains.The results showed that 211 samples of root rot diseased chili pepper plant were collected and 161 isolates were obtained.121 of these,75.16% of the total isolates,belonged to Fusarium which were the main pathogen for root rot of processing chili pepper in Xinjiang.80 isolates with pathogenicity were identified to class into two different species,e.g.Fusarium oxysporum and Fusarium solani.Fusarium oxysporum were 57 strains,accounted for 47.11%,Fusarium solani were 23 strains,accounted for 19.01%.

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