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Position: Home > Articles > Cloning and expression of transcription factor gene MrDREBA6 from Malus micromalus Journal of Fruit Science 2009,26 (6) 5-12

八棱海棠中转录因子基因MrDREBA6的克隆及表达分析

作  者：
付晓燕;彭日荷;章镇;乔玉山;周军
单  位：
南京农业大学园艺学院;扬州大学艺术学院;上海市农业科学院生物技术研究所
关键词：
八棱海棠;转录因子;RACE;DREB类;荧光定量PCR
摘  要：
DREB类基因是植物中重要的一类转录因子基因,其编码的产物广泛参与生长发育和各类生理过程。根据拟南芥和水稻DREB类基因序列,设计2个兼并引物,利用RACE法从八棱海棠cDNA文库中扩增出一编码DREB类转录因子的cDNA序列,命名为MrDREBA6。从cDNA序列、推测的氨基酸序列、进化树、功能域和分子结构模型进行预测和较为全面的分析,表明MrDREBA6属于DREB类转录因子的A6亚族,蛋白质三级结构与AtRAP2.4和AtERF1相似。荧光定量PCR分析MrDREBA6在各种处理下表达水平显示,MrDREBA6受到PEG、ABA、高盐和低温的诱导表达。另外,组织表达特异性检测表明,正常生长条件下MrDREBA6基因在叶中表达较高,在根和茎中的表达量略低。
译  名：
Cloning and expression of transcription factor gene MrDREBA6 from Malus micromalus
作  者：
FU Xiao-yan1,PENG Ri-he1,ZHANG Zhen2,QIAO Yu-shan2,ZHOU Jun2,ZHU Bo1,GAO Feng1,TIAN Yong-sheng1,ZHAO Wei1,XIONG Ai-sheng1,YAO Quan-hong1(1Biotechnology Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201106 China;2College of Horticulture,Nanjing Agricultural University,Nanjing,Jiangsu 210095 China)
关键词：
Malus micromalus Makino;Transcription factor;RACE;DREB family;Real-Time-Quantitative PCR
摘  要：
MrDREB gene is an important family of transcription factors in plant,encoding transcriptional regulators with a variety of functions involved in the developmental and physiological processes.Here,using two degenerate primers according to the sequences of DREB genes from Arabidopsis and rice,a cDNA encoding putative DREB protein was cloned from cDNA of Malus micromalus Makino by RACE,named as MrDREBA6.Then,cDNA and deduced amino acid sequence,phylogenetic tree,function domain and three-dimension structure were predicted and analyzed.MrDREBA6 from Malus micromalus Makino was classified into A6 subgroup of DREB family transcription factor.MrDREBA6,AtRAP2.4 and AtERF1 have similar three-dimension structure.The results of Real-Time-Quantitative PCR analysis for Malus micromalus Makino exposed to various treatments demonstrated that MrDREBA6 gene was induced by PEG,ABA,high-salinity and low-temperature stresses.Expression profiling analyses of MrDREBA6 in different organs indicated that MrDREBA6 gene was mainly expressed in leaf,and faintly expressed in root and stem under normal growth condition.

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