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Position: Home > Articles > Analysis on the Stability of 1RS in the Wheat-rye 1BL/1RS Translocation Lines Journal of Triticeae Crops 2006,26 (6) 16-20

小麦1BL/1RS易位系1RS分子标记位点稳定性分析

作  者:
苏亚蕊;张大乐;高安礼;李玉阁;李锁平
单  位:
河南大学河南省植物逆境生物学重点实验室
关键词:
小麦;黑麦;1BL/1RS易位系;分子标记
摘  要:
为检测小麦1BL/1RS易位系1RS位点的稳定性,利用1RS上11个标记位点的PCR特异引物对21个小麦1BL/1RS易位系进行了分子检测。结果表明,21个1BL/1RS易位系中,66.7%的品种1RS的11个标记位点较为稳定,扩增出标记位点特异性带,但有33.3%的品种部分标记位点出现特异性带的丢失或添加,具有不同程度的位点变异,位点变异率最高达45.5%。对于1RS上的11种PCR特异引物,引物NOR-R1和APR1.3可稳定扩增出黑麦特异带,是在小麦遗传背景中鉴别黑麦1RS较为可靠的分子标记。
译  名:
Analysis on the Stability of 1RS in the Wheat-rye 1BL/1RS Translocation Lines
作  者:
SU Ya-rui,ZHANG Da-le,GAO An-li,LI Yu-ge,LI Suo-ping(Lab.of Plant Stress Biology,Henan University,Kaifeng,Henan 475001,China)
关键词:
Wheat;Rye;1BL/1RS translocation lines;Specific primers
摘  要:
In order to identify the stability of 1RS in these wheat-rye 1BL/1RS translocation lines,11 1RS specific primer sets were used to detect the 1RS in 21 wheat-rye 1BL/1RS translocation lines.The results indicated that 66.7% of the 21 wheat-rye 1BL/1RS translocation lines had a stable 1RS and amplified the specific band.However,33.3% of the lines had some variations,there were some deletions and additions of the specific band in 1RS.The highest variation frequency is 45.5%.In addition,among the 11 1RS specific primers used in the experiment,only the primer sets NOR-R1 and APR(1.3) amplified the special band in all samples,which provided a useful means for molecular tagging of rye chromosomes 1RS in wheat genetic background.

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