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Position: Home > Articles > Integration of QTL mapping information in soybean symbiotic nodule-related traits and analysis of candidate genes Chinese Journal of Oil Crop Sciences 2017,39 (5) 580-588

大豆共生结瘤相关性状QTL定位信息整合及候选基因分析

作  者:
张艳娇;李长育;王锦辉;常慧琳;魏玮;马超;姜志浩;蒋洪蔚;刘春燕;尹振功;高山;金喜军;辛大伟;陈庆山
单  位:
黑龙江八一农垦大学农学院
关键词:
大豆;结瘤;Meta分析;Meta-QTL
摘  要:
目前基于大豆共生结瘤QTL定位的结果少、置信区间大,难以应用于实践中,因此整合前人研究结果、缩短置信区间具有重要意义。本研究针对大豆共生结瘤相关性状,搜集国内外已报道的48个QTL定位结果,利用Meta分析得到2个分别控制结瘤数目、结瘤大小和结瘤干鲜重的Meta-QTL。在区间内选择候选基因,利用野生大豆和栽培大豆验证候选基因在接种根瘤菌后的表达模式,明确候选基因与共生结瘤的关系。对Meta-QTL区间内的6个候选基因进行qRT-PCR检测及初步生物信息学分析,结果表明其中4个基因有可能为大豆共生结瘤性状的候选基因。本研究的实验方法对QTL定位区间内候选基因的确定和功能研究具有指导意义。
译  名:
Integration of QTL mapping information in soybean symbiotic nodule-related traits and analysis of candidate genes
作  者:
ZHANG Yan-jiao;LI Chang-yu;WANG Jin-hui;CHANG Hui-lin;WEI Wei;MA Chao;JIANG Zhi-hao;JIANG Hong-wei;LIU Chun-yan;YIN Zhen-gong;GAO Shan;JIN Xi-jun;XIN Da-wei;CHEN Qing-shan;Key Laboratory of Soybean Biology and Breeding Genetics of Chinese Agriculture Ministry Collgeg of Agricuture,Northeast Agricultural University;Heilongjiang Academy of Agricultural Sciences;Heilongjiang Bayi Agricultural University;
关键词:
soybean;;nodulation;;meta-analysis;;meta-QTL
摘  要:
Symbiotic nodulation in soybean is an important agronomic trait affecting soybean biological nitrogen fixation. Few QTLs for symbiotic nodulation with small confidence interval could be applied in practice. Our purpose was to integrate the results of previous studies and propose meta-QTL. Meta-analysis was carried out based on 48 QTLs about symbiotic nodule-related traits across 4 independent studies,and two meta-QTLs controlling nodule number,nodule size and nodule dry-weight were proposed. Six candidate genes were screened from the interval of meta-QTL and their expressional patterns were analyzed by qRT-PCR in cultivar soybean and wild soybean inoculated with rhizobia. Among them,4 genes may be involved in symbiotic nodulation in soybean. This two meta-QTLs should prove helpful in identification and functionality study of candidate genes associated with symbiotic nodule-related traits.

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