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当前位置: 首页 > 文章 > 替来他明诱导大鼠中枢神经系统c-fos基因的表达 畜牧兽医学报 2010,41 (7) 878-882
Position: Home > Articles > Tiletamine Induced c-fos Oncogene Expression within Central Nervous System of Rats Chinese Journal of Animal and Veterinary Sciences 2010,41 (7) 878-882

替来他明诱导大鼠中枢神经系统c-fos基因的表达

作  者：
卢德章;范宏刚;于世明;张栾松;马昆;马海鹍;王洪斌
单  位：
东北农业大学动物医学学院
关键词：
替来他明;c-fos基因;中枢神经系统
摘  要：
本研究旨在观察替来他明麻醉后大鼠中枢神经系统c-fos基因的表达,了解替来他明在中枢神经系统的作用部位,探讨替来他明对中枢神经系统的作用机制。72只SD大鼠,随机分为生理盐水组、给药后10、30、60、90、100、120和180 min组,每组9只。腹腔注射替来他明50 mg.kg-1后,分别于给药前(生理盐水组)和给药后10、30、60、90、100、120和180 min经4%多聚甲醛(0.1 mol.L-1,PB,pH7.4)灌注后,取脑,将脑分为大脑皮层、海马、丘脑、小脑、脑干5个脑区,置于20%蔗糖溶液中24 h(4℃)脱水。冰冻切片,片厚10μm,按照Elivision法进行免疫组化染色,观察鉴别Fos阳性神经元并做阳性神经元计数。结查显示,对照组中仅发现少量Fos阳性神经元,试验组中Fos阳性神经元在大脑皮层、海马、丘脑、小脑、脑干内都有表达。替来他明腹腔注射10 min后Fos阳性神经元表达开始增加,60 min表达至高峰,90 min表达下降,180 min下降至基线水平,与给药前相比无显著性差异(P>0.05)。结果提示,替来他明能诱导大鼠大脑皮层、海马、丘脑、小脑、脑干c-fos基因的表达,大脑皮层、海马、丘脑、小脑和脑干是替来他明的作用位点。
译  名：
Tiletamine Induced c-fos Oncogene Expression within Central Nervous System of Rats
作  者：
LU De-zhang,FAN Hong-gang,YU Shi-ming,ZHANG Luan-song,MA Kun,MA Hai-kun,WANG Hong-bin(College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China)
关键词：
Tiletamine;c-fos oncogene;central nervous system
摘  要：
To investigate the distribution of c-fos oncogene expression in rats central nervous system following tiletamine anesthesia.Tiletamine action sites in CNS were studied,and general anesthetic mechanisms were also discussed.72 SD rats were randomly divided into 8 groups.At 0,10,30,60,90,100,120 and 180 min after given tiletamine 50 mg·kg-1 intraperitoneally,rats were perfused with 4% Paraformaldehyde(0.1 mol·L-1,PB,pH 7.4).The whole brain was separated into cerebral cortex,hippocampus,thalamus,cerebellum and brain stem.Then these brain tissues were dehydrated 24 h by 20% sucrose(4 ℃).Cryosections were 10 μm and Elivision Immunohistochemical technique for Fos-protein was used to observe and count on the Fos-like immunoreactive neurons.In normal saline group,there was few Fos-like immunoreactive neurons appeared.And Fos-like immunoreactive neurons were observed in cerebral cortex,hippocampus,thalamus,cerebellum and brain stem in experimental groups.Fos-like immunoreactive neurons expression increased at 10 min after tiletamine administrated by intraperitoneal injection,peaked at 60 min and decreased at 90 min.At 180 min after injection,Fos-like immunoreactive neurons recovered to base level,which had no significant difference compared with that before given tiletamine(P>0.05).The results showed that tiletamine induce expression of c-fos oncogene in the rats′ cerebral cortex,hippocampus,thalamus,cerebellum and brain stem.And the cerebral cortex,hippocampus,thalamus,cerebellum and brain stem at the action sites of tiletamine.

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