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当前位置: 首页 > 文章 > β-氨基丁酸诱导辣椒细胞色素P450基因的克隆及其序列分析 园艺学报 2007,34 (3) 637-643
Position: Home > Articles > Molecular Cloning and Sequence Analysis of Cytochrome P450 in Pepper (Capsicum annuum L.) Induced by β-aminobutyric Acid (BABA) Acta Horticulturae Sinica 2007,34 (3) 637-643

β-氨基丁酸诱导辣椒细胞色素P450基因的克隆及其序列分析

作  者：
戴素明;周程爱;谢丙炎;肖启明;杨宇红;尹丽蓉
单  位：
湖南农业大学生物安全科技学院;中国农业科学院蔬菜花卉研究所
关键词：
辣椒;细胞色素;β-氨基丁酸;生物信息;防御反应
摘  要：
用RT-PCR和RACE方法,从β-氨基丁酸(β-aminobutyric acid,BABA)诱导辣椒叶片的基因表达体系中克隆出CYP92A的全长cDNA序列。在BABA处理后,CYP92A基因以一种快速而短暂的方式作出应答。生物信息学分析表明,该基因编码一条509个氨基酸残基的多肽,含有一段细胞色素P450保守的血红素结合区域,由此CYP92A被确定是细胞色素P450。通过系统进化分析,CYP92A属于多基因家族P450的92A亚家族。结合已报道92A亚家族成员的研究,推测CYP92A参与植物的防御反应。通过生物信息学分析蛋白结构,对CYP92A催化反应特性进行了讨论。为进一步探讨BABA的诱导机理奠定了基础。
译  名：
Molecular Cloning and Sequence Analysis of Cytochrome P450 in Pepper (Capsicum annuum L.) Induced by β-aminobutyric Acid (BABA)
作  者：
DAI Su-ming1,2,ZHOU Cheng-ai 1,2,XIE Bing-yan1,XIAO Qi-ming2,YANG Yu-hong1,and YIN Li-rong1,2(1Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;2College of Plant Protection,Hunan Agricultural University,Changsha 410128,China)
关键词：
Capsicum annuum L.;Cytochrome;BABA;Bioinformatics;Plant defence response
摘  要：
The complete CYP92A was amplified by RT-PCR and RACE,which expressed in pepper(Capsicum annuum L.) leaf was rapidly and transiently induced by β-aminobutyric acid(BABA).The analysis of bioinformatics showed that CYP92A gene encoded a 509-amino acid protein which contained a conserve heme-binding region of cytochrome P450.Therefore,it was confirmed that CYP92A is cytochrome P450.By phylogenetic analysis,it was belived to belong to 92A sub-family of P450.According to researches of other 92A sub-family genes reported,it is speculated conferred that CYP92A may play a role in plant defence response.Based on bioinformatics analysis of protein struction,the property of CYP92A in catalytic reaction was also discussed in this paper.This study had significance for research on indued-resistance mechanisms of BABA.

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