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Acta Agriculturae Boreali-occidentalis Sinica
2016,25
(8)
1195-1200
一株抗铅细菌的分离鉴定与抗铅性能研究
作 者：
瞿佳;赵玲侠;孙晓宇;陈锐;路鹏鹏;沈卫荣
单 位：
陕西省微生物研究所微生物资源中心
关键词：
抗铅微生物;产碱菌;鉴定;抗铅性能
摘 要：
为获得可应用于农田土壤重金属污染治理的铅(Pb)降解功能菌株,从陕西商洛铅锌矿区周边农田土壤中分离筛选出1株抗铅细菌,命名为PS8,对其形态特征、生理生化、16SrDNA同源性及抗铅性能进行研究。结果表明,菌株PS8为革兰氏阴性细菌,菌落呈圆形、淡黄色;最适生长温度为30℃,最适pH为7.0;可还原亚硝酸盐,氧化酶、接触酶反应呈阳性;与粪产碱菌Alcaligenes faecali高度同源;在Pb2+浓度为0.5和1.0mmol/L时,去除率分别为99.08%和97.24%。菌株PS8对重金属铅具有高抗性和较强降解能力,是高效、环境友好的土壤重金属铅污染微生物修复菌株。
译 名：
Isolation,Identification of a Lead-resistant Strain and Effect of Lead Removal
作 者：
QU Jia;ZHAO Lingxia;SUN Xiaoyu;CHEN Rui;LU Pengpeng;SHEN Weirong;Microbial Resource Center,Microbiology Insititute of Shaanxi;
关键词：
Lead resistant bacteria;;Alcaligenes;;Identification;;Removal capacity
摘 要：
In order to obtain resistant bacteria for removing heavy metal in polluted cultivated field,a lead-resistant strain,named PS8,was isolated from metal-polluted soil in a cultivated field near lead-zinc mining area in Shangluo,Shaanxi province.Based on 16 SrDNA sequence analysis,PS8 was highly homologous to Alcaligenes faecali by studying the morphological,physiological-biochemical properties,the results showed that PS8 was gram negative,circle-shaped,yellow color and its most suitable growth condition was 30℃ pH 7.0.In addition,Nitrite reduction test,oxidase test and catalase test were all positive.The Pb2+removal effect of PS8 was 99.08% and 97.24% respectively in 0.5mmol/L and 1.0mmol/L lead concentrations.In conclusion,PS8 is an effective,environmental-friendly strain and have remarkable value to remediate heavy metal contaminated soil.
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