Qin Bao;Haiyan Jiang;Yingli Liu;Yuan Yan;Jiang WenLa

Guangxi Traditional Chinese Medical University,Nanning,Guangxi 530001,China;Guangxi Traditional Chinese Medical University,Nanning,Guangxi 530001,Chin

rhizoma curcumae;germacrone;volatile oil;different producing area;the contents;lingsha

[Objective] To compare the contents of volatile oil and germacrone in RHIZOMA CURCUMAE from different producing area such as Lingshan,Guiping and Guigang in Guangxi Province.[Method] The volatile oil was extracted from RHIZOMA CURCUMAE by steam distillation.The content of germacrone was determined by high performance liquid chromatography method (HPLC),and its chromatograph conditions were as follows:acetonitrile-water (V/V,70:30),flow rate of 1 ml/min,column temperature of 35 ℃,detection wavelength of 215 nm and sampling volume of 10 μl.[Result] The germacrone presented a good linear relation between the detection concentration of 0.022 3 and 0.139 8 mg/ml.Contents of volatile oil and germacrone in RHIZOMA CURCUMAE were varied for different producing area.[Conclusion] The contents of volatile oil and germacrone in RHIZOMA CURCUMAE from Lingshan were higher than that in other 8 areas.