中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > Overexpression of PbrGA2ox1 enhances pear drought tolerance through the regulation of GA3-inhibited 3-inhibited reactive oxygen species detoxification and abscisic acid signaling 农业科学学报 (英文) 2024,23 (9)
Position: Home > Articles > Overexpression of PbrGA2ox1 enhances pear drought tolerance through the regulation of GA3-inhibited 3-inhibited reactive oxygen species detoxification and abscisic acid signaling Journal of Integrative Agriculture 2024,23 (9)

Overexpression of PbrGA2ox1 enhances pear drought tolerance through the regulation of GA3-inhibited 3-inhibited reactive oxygen species detoxification and abscisic acid signaling

作  者：
Guo, Guoling;Zhang, Haiyan;Dong, Weiyu;Xu, Bo;Wang, Youyu;Zhao, Qingchen;Liu, Lun;Tang, Xiaomei;Liu, Li;Ye, Zhenfeng;Heng, Wei;Zhu, Liwu;Jia, Bing
单  位：
Anhui Agr Univ, Sch Hort, Hefei 230036, Peoples R China;Anhui Wanbang Pharmaceut Technol Co Ltd, Hefei 230036, Peoples R China;Hunan Suncas Agr Sci Co Ltd, Changsha 410016, Peoples R China;Anhui Agr Univ, Sci & Technol Dept, Hefei 230036, Peoples R China
关键词：
pear;gibberellin;drought stress;PbrGA2ox1;reactive oxygen species;abscisic acid
摘  要：
Drought stress is a devastating natural disaster driven by the continuing intensification of global warming, which seriously threatens the productivity and quality of several horticultural crops, including pear. Gibberellins (GAs) play crucial roles in plant growth, development, and responses to drought stress. Previous studies have shown significant reductions of GA levels in plants under drought stress; however, our understanding of the intrinsic regulation mechanisms of GA-mediated drought stress in pear remains very limited. Here, we show that drought stress can impair the accumulation of bioactive GAs (BGAs), and subsequently identified PbrGA2ox1 as a chloroplast-localized GA deactivation gene. This gene was significantly induced by drought stress and abscisic acid (ABA) treatment, but was suppressed by GA3 3 treatment. PbrGA2ox1- overexpressing transgenic tobacco plants ( Nicotiana benthamiana) ) exhibited enhanced tolerance to dehydration and drought stresses, whereas knockdown of PbrGA2ox1 in pear ( Pyrus betulaefolia) ) by virus-induced gene silencing led to elevated drought sensitivity. Transgenic plants were hypersensitive to ABA, and had a lower BGAs content, enhanced reactive oxygen species (ROS) scavenging ability, and augmented ABA accumulation and signaling under drought stress compared to wild-type plants. However, the opposite effects were observed with PbrGA2ox1 silencing in pear. Moreover, exogenous GA3 3 treatment aggravated the ROS toxic effect and restrained ABA synthesis and signaling, resulting in the compromised drought tolerance of pear. In summary, our results shed light on the mechanism by which BGAs are eliminated in pear leaves under drought stress, providing further insights into the mechanism regulating the effects of GA on the drought tolerance of plants.

相似文章

PDF下载 PDF预览
导出引用
计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46