Position: Home > Articles > Extraction and Determination of Total Flavonoids from Ficus beecheyana Radix
Journal of Anhui Agricultural Sciences
2013
(2)
143-145
天仙果根总黄酮提取工艺研究
作 者：
王喜周;应跃跃;张昊;姚晓伟;陈学智
单 位：
丽水学院医学院;景宁县人民医院;丽水市质量技术监督检测院
关键词：
天仙果(Ficuserecta Thunb.var.beecheyana(Hook.et Arn.)King);总黄酮;提取工艺;紫外分光光度法
摘 要：
[目的]优选天仙果根中总黄酮的超声辅助提取工艺。[方法]以总黄酮的含量为指标,采用单因素试验考察乙醇浓度、料液比、提取时间和提取次数对天仙果根部总黄酮得率的影响,并采用正交试验优选天仙果根总黄酮的超声辅助提取工艺。[结果]4个因素对天仙果根部总黄酮提取得率的影响大小顺序为:料液比>乙醇浓度>提取时间>提取次数;最佳提取工艺为:提取溶剂乙醇的浓度为50%,料液比1∶20(W/V,g/ml,下同),提取2次,每次60 min;在此条件下,天仙果根部总黄酮得率为27.83 mg/g。[结论]提取工艺合理,测定方法简单、快速、准确,为天仙果深度开发提供了科学依据。
译 名：
Extraction and Determination of Total Flavonoids from Ficus beecheyana Radix
作 者：
WANG Xi-zhou et al(Medical College of Lishui University,Lishui,Zhejiang 323000)
关键词：
Ficus beecheyana;Total flavonoids;Extraction technique;UV spectrophotometry
摘 要：
[Objective] To optimum ultrasonic-assisted extraction technique of total flavonoids in radix of Ficus beecheyana.[Method] Effects of ethanol concentration,solid-liquid ratio,extraction time and times on yield of total flavonoids were investigated by using orthogonal test,and the contents were determined by UV spectrophotometry.[Result] The order of influencing factors is: solid-liquid ratio> ethanol concentration> extraction time> extraction times;the optimal extraction technique is: ethanol concentration 50%,solid-liquid ratio 1∶20(W/V,g/ml,the same as follows),extraction 2 times and extraction time 60 min.Under these optimum conditions,the extraction efficiency of total flavonoids reached as high as 27.83 mg/g.[Conclusion] The extraction technique is proper,the determination method is simple,rapid and accurate,which will provide a scientific basis for deeply utilization of Ficus beecheyana.
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